• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP2R5D Polyclonal antibody

PPP2R5D Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12068-1-AP

Synonyms

PP2A B subunit isoform R5-delta, PP2A B subunit isoform PR61-delta, PP2A B subunit isoform B'-delta, PP2A B subunit isoform B56-delta, PP2A B subunit isoform B delta

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Y79 cells using PPP2R5D Polyclonal antibody (12068-1-AP)

    WB analysis of Y79 using 12068-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12068-1-AP (PPP2R5D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PPP2R5D Polyclonal antibody (12068-1-AP)

    WB analysis of human brain using 12068-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12068-1-AP (PPP2R5D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using PPP2R5D Polyclonal antibody (12068-1-AP)

    IP experiment of Y79 using 12068-1-AP

    IP result of anti-PPP2R5D (IP:12068-1-AP, 4ug; Detection:12068-1-AP 1:400) with Y79 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PPP2R5D Polyclonal antibody (12068-1-AP)

    IHC staining of human stomach using 12068-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12068-1-AP (PPP2R5D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach tissue using PPP2R5D Polyclonal antibody (12068-1-AP)

    IHC staining of human stomach using 12068-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12068-1-AP (PPP2R5D antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Positive WB detected inY79 cells, human brain tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12068-1-AP targets PPP2R5D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R5D fusion protein Ag2705 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 2, regulatory subunit B', delta isoform
Calculated molecular weight 602 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC010692
Gene symbol PPP2R5D
Gene ID (NCBI) 5528
RRIDAB_2299977
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP2R5D antibody 12068-1-APDownload protocol
IHC protocol for PPP2R5D antibody 12068-1-APDownload protocol
IP protocol for PPP2R5D antibody 12068-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network

Authors - Cuirong Guan
View Article
mouseWB

Biomedicines

Unmasking Protein Phosphatase 2A Regulatory Subunit B as a Crucial Factor in the Progression of Dilated Cardiomyopathy

Authors - Fang Lin
  • KD Validated
View Article