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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using PPP3CA-specific Polyclonal antibody (55147-1-AP)

    WB analysis of mouse brain using 55147-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55147-1-AP (PPP3CA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using PPP3CA-specific Polyclonal antibody (55147-1-AP)

    WB analysis of mouse pancreas using 55147-1-AP

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 55147-1-AP (PPP3CA-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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PPP3CA-specific Polyclonal antibody

PPP3CA-specific Polyclonal Antibody for WB, ELISA
Cat No. 55147-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

Calcineurin A alpha, CALN, CALNA, CALNA1, CAM PRP catalytic subunit, CCN1, CNA, CNA1, PPP2B, PPP3CA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inmouse brain tissue, mouse pancreas tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55147-1-AP targets PPP3CA-specific in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Calculated molecular weight 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberNM_000944
Gene Symbol PPP3CA
Gene ID (NCBI) 5530
RRIDAB_10858786
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ08209
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation. This antibody is specific to PPP3CA.

Protocols

Product Specific Protocols
WB protocol for PPP3CA-specific antibody 55147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols