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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP3CA Monoclonal antibody (68163-1-Ig)

    WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPP3CA Monoclonal antibody (68163-1-Ig)

    WB analysis using 68163-1-Ig (same clone as 68163-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68163-1-Ig (PPP3CA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of rat brain tissue using PPP3CA Monoclonal antibody (68163-1-Ig)

    IP experiment of rat brain using 68163-1-Ig (same clone as 68163-1-PBS)

    IP result of anti-PPP3CA (IP:68163-1-Ig, 5ug; Detection:68163-1-Ig 1:4000) with rat brain tissue lysate 1120 ug. This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPP3CA Monoclonal antibody (68163-1-Ig)

    IHC staining of mouse skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PPP3CA Monoclonal antibody (68163-1-Ig)

    IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PPP3CA Monoclonal antibody (68163-1-Ig)

    IHC staining of human thyroid cancer using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPP3CA Monoclonal antibody (68163-1-Ig)

    IHC staining of mouse skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using PPP3CA Monoclonal antibody (68163-1-Ig)

    IHC staining of rat skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat skeletal muscle tissue using PPP3CA Monoclonal antibody (68163-1-Ig)

    IHC staining of rat skeletal muscle using 68163-1-Ig (same clone as 68163-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 68163-1-Ig (PPP3CA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68163-1-PBS in a different storage buffer formulation.

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PPP3CA Monoclonal antibody, PBS Only

PPP3CA Monoclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 68163-1-PBS
Clone No.2A5C1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CALNA, CALNA1, CALN, Calmodulin-dependent calcineurin A subunit alpha isoform, Calcineurin A alpha

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68163-1-PBS targets PPP3CA in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PPP3CA fusion protein Ag32642 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
Calculated molecular weight 59 kDa
Observed molecular weight59 kDa
GenBank accession numberBC025714
Gene Symbol PPP3CA
Gene ID (NCBI) 5530
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ08209
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PPP3CA, also called calcineurin Aα, is the only serine/threonine protein phosphatase under the control of Ca2+/calmodulin, and plays a critical role in the coupling of Ca2+ signals to cellular responses. Ca2+ signaling plays a central role in hypertrophic growth of cardiac and skeletal muscle in response to mechanical load and a variety of signals. Therefore, PPP3CA plays an important role in muscle differentiation, especially in muscle fiber type conversion. It is also involved in osteoclast regulation, regulating bone formation through an effect on osteoblast differentiation.