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KD/KO Validated

PPP3CB Polyclonal antibody

Name: PPP3CB Polyclonal antibody
Brand: Proteintech
SKU: 13340-1-AP

PPP3CB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 13340-1-AP

Synonyms

CALNA2, Calmodulin-dependent calcineurin A subunit beta isoform, CNA beta, CAM-PRP catalytic subunit, CAM PRP catalytic subunit

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 13340-1-AP
WB result of PPP3CB antibody (13340-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP3CB transfected HEK-293 cells.
WB analysis using 13340-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13340-1-AP (PPP3CB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 13340-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 13340-1-AP (PPP3CB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 13340-1-AP
IP result of anti-PPP3CB (IP:13340-1-AP, 4ug; Detection:13340-1-AP 1:2000) with mouse brain tissue lysate 1680 ug.
IHC staining of mouse kidney using 13340-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 13340-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13340-1-AP (PPP3CB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of U-251 using 13340-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed U-251 cells using PPP3CB antibody (13340-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 13340-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse brain tissue, human heart tissue, HEK-293 cells, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	U-251 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

13340-1-AP targets PPP3CB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPP3CB fusion protein Ag4163 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
Calculated molecular weight	525 aa, 59 kDa
Observed molecular weight	59 kDa
GenBank accession number	BC028049
Gene symbol	PPP3CB
Gene ID (NCBI)	5532
RRID	AB_2284217
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP3CB antibody 13340-1-AP	Download protocol
IHC protocol for PPP3CB antibody 13340-1-AP	Download protocol
IF protocol for PPP3CB antibody 13340-1-AP	Download protocol
IP protocol for PPP3CB antibody 13340-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Gastroenterology Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1. Authors - Haojie Jin View Article
human	IP	Cell Death Differ The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma. Authors - Jialiang Liu View Article
human	WB	Oncogene Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells. Authors - Mengyi Lao KD Validated View Article
		Sci Rep Calcineurin regulates cyclin D1 stability through dephosphorylation at T286. Authors - Takahiro Goshima View Article
human	WB	Proteomics Analysis of EV71 infection progression using triple-SILAC-based proteomics approach. Authors - Hao-Yu Li View Article
mouse	WB	Eur J Pharmacol Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. Authors - Jiali Zheng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP3CB Polyclonal antibody

PPP3CB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 13340-1-AP

WB analysis using 13340-1-AP

WB analysis of human heart using 13340-1-AP

IP experiment of mouse brain using 13340-1-AP

IHC staining of mouse kidney using 13340-1-AP

IHC staining of mouse kidney using 13340-1-AP

IF Staining of U-251 using 13340-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.

The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Calcineurin regulates cyclin D1 stability through dephosphorylation at T286.

Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.