PPP3R2 Polyclonal antibody

Name: PPP3R2 Polyclonal antibody
Brand: Proteintech
SKU: 14005-1-AP

PPP3R2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 14005-1-AP

Synonyms

Calcineurin B like protein, Calcineurin BII, Calcineurin subunit B type 2, CBLP, CNBII, PPP3R2, PPP3RL

Validation Data Gallery

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AllWBIPIHC

WB analysis of mouse testis using 14005-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14005-1-AP (PPP3R2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 14005-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 14005-1-AP (PPP3R2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 14005-1-AP
IP result of anti-PPP3R2 (IP:14005-1-AP, 4ug; Detection:14005-1-AP 1:500) with mouse testis tissue lysate 4000 ug.
IHC staining of human testis using 14005-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14005-1-AP (PPP3R2 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human testis using 14005-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 14005-1-AP (PPP3R2 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human prostate cancer using 14005-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14005-1-AP (PPP3R2 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate cancer using 14005-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14005-1-AP (PPP3R2 antibody) at dilution of 1:50 (under 40x lens).

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Cat No. 14005-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse testis tissue
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human testis tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 2 publications below
IF	See 2 publications below

Product Information

14005-1-AP targets PPP3R2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PPP3R2 fusion protein Ag5097 相同性解析による交差性が予測される生物種
Full Name	protein phosphatase 3 (formerly 2B), regulatory subunit B, beta isoform
Calculated molecular weight	20 kDa
Observed molecular weight	20 kDa
GenBank accession number	BC030595
Gene symbol	PPP3R2
Gene ID (NCBI)	5535
RRID	AB_2168590
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP3R2 antibody 14005-1-AP	Download protocol
IHC protocol for PPP3R2 antibody 14005-1-AP	Download protocol
IP protocol for PPP3R2 antibody 14005-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Proc Natl Acad Sci U S A SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Authors - Haruhiko Miyata View Article
mouse	IHC	Cell Death Dis miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2. Authors - Xudong Zhang View Article
mouse	IF, IHC, WB	FASEB J Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs. Authors - Souvik Dey View Article
mouse	WB	Eur J Pharmacol Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway. Authors - Jiali Zheng View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PPP3R2 Polyclonal antibody

PPP3R2 Polyclonal Antibody for WB, IP, IHC, ELISA

Validation Data Gallery

WB analysis of mouse testis using 14005-1-AP

WB analysis of mouse testis using 14005-1-AP

IP experiment of mouse testis using 14005-1-AP

IHC staining of human testis using 14005-1-AP

IHC staining of human testis using 14005-1-AP

IHC staining of human prostate cancer using 14005-1-AP

IHC staining of human prostate cancer using 14005-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

miR-146a facilitates osteoarthritis by regulating cartilage homeostasis via targeting Camk2d and Ppp3r2.

Roles of glycogen synthase kinase 3 alpha and calcineurin in regulating the ability of sperm to fertilize eggs.

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.