• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HeLa cells using PPP4R1 Polyclonal antibody (27805-1-AP)

    WB analysis of HeLa using 27805-1-AP

    WB result of PPP4R1 antibody (27805-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP4R1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using PPP4R1 Polyclonal antibody (27805-1-AP)

    WB analysis using 27805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27805-1-AP (PPP4R1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPP4R1 Polyclonal antibody (27805-1-AP)

    WB analysis using 27805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27805-1-AP (PPP4R1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using PPP4R1 Polyclonal antibody (27805-1-AP)

    IP experiment of HEK-293T using 27805-1-AP

    IP result of anti-PPP4R1 (IP:27805-1-AP, 4ug; Detection:27805-1-AP 1:2000) with HEK-293T cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPP4R1 Polyclonal antibody (27805-1-AP)

    IHC staining of human liver cancer using 27805-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27805-1-AP (PPP4R1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPP4R1 Polyclonal antibody (27805-1-AP)

    IHC staining of human liver cancer using 27805-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27805-1-AP (PPP4R1 antibody) at dilution of 1:200 (under 40x lens).

☆ 3/31（月）まで！二次抗体 & FlexAbleが30%オフ！ >> 免疫アッセイサポートキャンペーン

  • Featured Product
  • KD/KO Validated

PPP4R1 Polyclonal antibody

PPP4R1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 27805-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Synonyms

MEG1, PP4(Rmeg), PP4R1, PPP4R1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 700 μg/ml
20 μL, 700 μg/ml
150 μL, 700 μg/ml

¥67,000/ 150 μL

本製品の国内在庫をすぐに見る（表示に数秒かかります）

【全製品対象☆】小さなギフトがついてくる！春の特典キャンペーン開催中

他社製品の代替品をお探しですか？『抗体代替品／同等品検索システム』でお調べいただけます

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HEK-293 cells, A431 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27805-1-AP targets PPP4R1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP4R1 fusion protein Ag26405 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 4, regulatory subunit 1
Calculated molecular weight 107 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC060829
Gene Symbol PPP4R1
Gene ID (NCBI) 9989
RRIDAB_2880978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TF05
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP4R1 antibody 27805-1-APDownload protocol
IHC protocol for PPP4R1 antibody 27805-1-APDownload protocol
IP protocol for PPP4R1 antibody 27805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Cancer Lett

PP4R1 promotes glycolysis and gallbladder cancer progression through facilitating ERK1/2 mediated PKM2 nuclear translocation

Authors - Zhiqiang He
View Article
humanWB,IP

Cell Death Differ

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner

Authors - Weimin Xu
View Article