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  • Featured Product
  • KD/KO Validated

PPP4R1 Polyclonal antibody

PPP4R1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27805-1-AP

Synonyms

MEG1, PP4(Rmeg), PP4R1, PPP4R1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPP4R1 Polyclonal antibody (27805-1-AP)

    WB analysis using 27805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27805-1-AP (PPP4R1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PPP4R1 Polyclonal antibody (27805-1-AP)

    WB analysis of HeLa using 27805-1-AP

    WB result of PPP4R1 antibody (27805-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP4R1 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using PPP4R1 Polyclonal antibody (27805-1-AP)

    WB analysis using 27805-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27805-1-AP (PPP4R1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293T cells using PPP4R1 Polyclonal antibody (27805-1-AP)

    IP experiment of HEK-293T using 27805-1-AP

    IP result of anti-PPP4R1 (IP:27805-1-AP, 4ug; Detection:27805-1-AP 1:2000) with HEK-293T cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPP4R1 Polyclonal antibody (27805-1-AP)

    IHC staining of human liver cancer using 27805-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27805-1-AP (PPP4R1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PPP4R1 Polyclonal antibody (27805-1-AP)

    IHC staining of human liver cancer using 27805-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27805-1-AP (PPP4R1 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HEK-293 cells, A431 cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27805-1-AP targets PPP4R1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP4R1 fusion protein Ag26405 相同性解析による交差性が予測される生物種
Full Name protein phosphatase 4, regulatory subunit 1
Calculated molecular weight 107 kDa
Observed molecular weight 120-130 kDa
GenBank accession numberBC060829
Gene symbol PPP4R1
Gene ID (NCBI) 9989
RRIDAB_2880978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PPP4R1 antibody 27805-1-APDownload protocol
IHC protocol for PPP4R1 antibody 27805-1-APDownload protocol
IP protocol for PPP4R1 antibody 27805-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Cancer Lett

PP4R1 promotes glycolysis and gallbladder cancer progression through facilitating ERK1/2 mediated PKM2 nuclear translocation

Authors - Zhiqiang He
View Article
humanWB,IP

Cell Death Differ

AMBRA1 promotes intestinal inflammation by antagonizing PP4R1/PP4c mediated IKK dephosphorylation in an autophagy-independent manner

Authors - Weimin Xu
View Article