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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using PPRC1 Polyclonal antibody (25429-1-AP)

    WB analysis of NIH/3T3 using 25429-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25429-1-AP (PPRC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPRC1 Polyclonal antibody (25429-1-AP)

    WB analysis using 25429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25429-1-AP (PPRC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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PPRC1 Polyclonal antibody

PPRC1 Polyclonal Antibody for WB, ELISA
Cat No. 25429-1-AP
Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

KIAA0595, PGC 1 related coactivator, PPRC1, PRC, RP11 302K17.6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inNIH/3T3 cells, C6 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

25429-1-AP targets PPRC1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPRC1 fusion protein Ag11940 相同性解析による交差性が予測される生物種
Full Name peroxisome proliferator-activated receptor gamma, coactivator-related 1
Calculated molecular weight 1542 aa, 165 kDa
Observed molecular weight 177 kDa
GenBank accession numberBC063806
Gene Symbol PPRC1
Gene ID (NCBI) 23082
RRIDAB_2880074
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5VV67
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peroxisome proliferator-activated receptor gamma, co-activator-related 1 (Pprc1) is the third member of the Pgc1 family, which contributes to mitochondrial biogenesis and orchestrates response to metabolic stress by promoting the expression of multiple genes specifying inflammation, proliferation, and metabolic reprogramming.

Protocols

Product Specific Protocols
WB protocol for PPRC1 antibody 25429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Res

PGC-1α degradation suppresses mitochondrial biogenesis to confer radiation resistance in glioma

Authors - Mengjie Zhao
View Article
humanWB

Sci Rep

CRIP1 inhibits cutaneous melanoma progression through TFAM-mediated mitochondrial biogenesis

Authors - Jianqiang Wu
View Article