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  • Featured Product
  • KD/KO Validated

PPT1 Polyclonal antibody

PPT1 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 29653-1-AP

Synonyms

CLN1, INCL, Palmitoyl protein hydrolase 1, PPT, PPT 1, PPT1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPT1 Polyclonal antibody (29653-1-AP)

    WB analysis using 29653-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29653-1-AP (PPT1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PPT1 Polyclonal antibody (29653-1-AP)

    WB analysis of A549 using 29653-1-AP

    WB result of PPT1 antibody (29653-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

  • Western Blot (WB) analysis of A549 cells using PPT1 Polyclonal antibody (29653-1-AP)

    WB analysis of A549 using 29653-1-AP

    WB result of PPT1 antibody (29653-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPT1 Polyclonal antibody (29653-1-AP)

    IHC staining of human kidney using 29653-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PPT1 Polyclonal antibody (29653-1-AP)

    IHC staining of human kidney using 29653-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29653-1-AP (PPT1 antibody) at dilution of 1:400 (under 10x lens). Heat-mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PPT1 Polyclonal antibody (29653-1-AP)

    IF Staining of mouse testis using 29653-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PPT1 antibody (29653-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, HeLa cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29653-1-AP targets PPT1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPT1 fusion protein Ag31240 相同性解析による交差性が予測される生物種
Full Name palmitoyl-protein thioesterase 1
Calculated molecular weight 34 kDa
Observed molecular weight32-34 kDa
GenBank accession numberBC008426
Gene symbol PPT1
Gene ID (NCBI) 5538
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

Protocols

Product Specific Protocols
WB protocol for PPT1 antibody 29653-1-APDownload protocol
IHC protocol for PPT1 antibody 29653-1-APDownload protocol
IF protocol for PPT1 antibody 29653-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Cell Rep Med

Targeting phenylpyruvate restrains excessive NLRP3 inflammasome activation and pathological inflammation in diabetic wound healing

Authors - Dongming Lv
  • KD Validated
View Article