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  • Featured Product
  • KD/KO Validated

PPT1 Monoclonal antibody, PBS Only

PPT1 Monoclonal Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rabbit

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A1A3

Cat no : 67699-1-PBS

Synonyms

Palmitoyl-protein thioesterase 1, Palmitoyl-protein hydrolase 1, INCL, EC:3.1.2.22, CLN1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PPT1 Monoclonal antibody (67699-1-Ig)

    WB analysis using 67699-1-Ig (same clone as 67699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPT1 Monoclonal antibody (67699-1-Ig)

    WB analysis using 67699-1-Ig (same clone as 67699-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PPT1 Monoclonal antibody (67699-1-Ig)

    WB analysis of A549 using 67699-1-Ig (same clone as 67699-1-PBS)

    WB result of PPT1 antibody (67699-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells. This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig heart tissue using PPT1 Monoclonal antibody (67699-1-Ig)

    WB analysis of pig heart using 67699-1-Ig (same clone as 67699-1-PBS)

    pig heart tissue were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PPT1 Monoclonal antibody (67699-1-Ig)

    IF Staining of mouse testis using 67699-1-Ig (same clone as 67699-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PPT1 antibody (67699-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67699-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67699-1-PBS targets PPT1 in WB, IF-P, Indirect ELISA applications and shows reactivity with human, pig, rabbit samples.

Tested Reactivity human, pig, rabbit
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PPT1 fusion protein Ag30550 相同性解析による交差性が予測される生物種
Full Name palmitoyl-protein thioesterase 1
Calculated molecular weight 34 kDa
Observed molecular weight32-34 kda
GenBank accession numberBC008426
Gene symbol PPT1
Gene ID (NCBI) 5538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).