PR Polyclonal antibody
PR Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (2)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 25871-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | T-47D cells |
| Positive IHC detected in | human breast cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
| IHC | See 14 publications below |
| IF | See 1 publications below |
Product Information
25871-1-AP targets PR in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PR fusion protein Ag22986 相同性解析による交差性が予測される生物種 |
| Full Name | progesterone receptor |
| Calculated molecular weight | 933 aa, 99 kDa |
| Observed molecular weight | 80 kDa, 110-115 kDa |
| GenBank accession number | NM_000926 |
| Gene symbol | Progesterone Receptor |
| Gene ID (NCBI) | 5241 |
| RRID | AB_2880277 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PGR, also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PR antibody 25871-1-AP | Download protocol |
| IHC protocol for PR antibody 25871-1-AP | Download protocol |
| IF protocol for PR antibody 25871-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance. | ||
Nucleic Acids Res ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD | ||
Acta Neuropathol Increased prevalence of granulovacuolar degeneration in C9orf72 mutation. | ||
Int J Mol Sci The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats | ||
Front Endocrinol (Lausanne) Effect of acupuncture on the opening time of implantation window and endometrial receptivity in controlled ovarian hyperstimulation rats during peri-implantation period | ||
Front Oncol Pan-Cancer Prognostic Role and Targeting Potential of the Estrogen-Progesterone Axis. |
