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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRAME Polyclonal antibody (11438-1-AP)

    WB analysis using 11438-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11438-1-AP (PRAME antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using PRAME Polyclonal antibody (11438-1-AP)

    IP experiment of K-562 using 11438-1-AP

    IP result of anti-PRAME (IP:11438-1-AP, 4ug; Detection:11438-1-AP 1:300) with K-562 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PRAME Polyclonal antibody (11438-1-AP)

    IHC staining of human lung cancer using 11438-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11438-1-AP (PRAME antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PRAME Polyclonal antibody (11438-1-AP)

    IHC staining of human lung cancer using 11438-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11438-1-AP (PRAME antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PRAME Polyclonal antibody

PRAME Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 11438-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, ELISA

Synonyms

MAPE, OIP 4, OIP4, Opa interacting protein 4, PRAME

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11438-1-AP targets PRAME in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRAME fusion protein Ag1906 相同性解析による交差性が予測される生物種
Full Name preferentially expressed antigen in melanoma
Calculated molecular weight 509 aa, 58 kDa
Observed molecular weight 50-58 kDa
GenBank accession numberBC014074
Gene Symbol PRAME
Gene ID (NCBI) 23532
RRIDAB_2169351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78395
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PRAME (preferentially expressed antigen of melanoma) gene was previously shown to be overexpressed in ovarian/primary peritoneal serous carcinoma compared with malignant mesothelioma using gene expression arrays. It is considered a melanocyte differentiation antigen which is overexpressed in both solid and hematologic tumors. In normal tissue, a very low level of PRAME expression is found in normal testis, adrenals, ovary and endometrium. A high level of PRAME expression has been reported for several solid tumors, including ovarian cancer, breast cancer, lung cancer and melanomas, medulloblastoma, sarcomas, head and neck cancers, neuroblastoma, renal cancer, and Wilms'tumor. As a nuclear transcriptional repressor protein, PRAME binds to retinoic acid receptor a, thereby inhibiting retinoic acid induced differentiation, growth arrest, and apoptosis.

Protocols

Product Specific Protocols
WB protocol for PRAME antibody 11438-1-APDownload protocol
IHC protocol for PRAME antibody 11438-1-APDownload protocol
IP protocol for PRAME antibody 11438-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols