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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

PRAS40 Polyclonal antibody

PRAS40 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21097-1-AP

Synonyms

AKT1S1, AKTS1, Lobe, PRAS40, Proline rich AKT1 substrate 1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PRAS40 Polyclonal antibody (21097-1-AP)

    WB analysis of MCF-7 using 21097-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRAS40 Polyclonal antibody (21097-1-AP)

    WB analysis of HeLa using 21097-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21097-1-AP (PRAS40 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PRAS40 Polyclonal antibody (21097-1-AP)

    IHC staining of human liver using 21097-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PRAS40 Polyclonal antibody (21097-1-AP)

    IHC staining of human liver using 21097-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21097-1-AP (PRAS40 antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRAS40 Polyclonal antibody (21097-1-AP)

    IF Staining of HeLa using 21097-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 21097-1-AP (PRAS40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21097-1-AP is PRAS40 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRAS40 fusion protein Ag15355 相同性解析による交差性が予測される生物種
Full Name AKT1 substrate 1 (proline-rich)
Calculated molecular weight 27 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC051844
Gene symbol PRAS40
Gene ID (NCBI) 84335
RRIDAB_2878811
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRAS40 antibody 21097-1-APDownload protocol
IHC protocol for PRAS40 antibody 21097-1-APDownload protocol
IF protocol for PRAS40 antibody 21097-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

Sexually dimorphic extracellular vesicle responses after chronic spinal cord injury are associated with neuroinflammation and neurodegeneration in the aged brain

Authors - Yun Li
View Article
humanWB

Front Oncol

The Molecular Landscape and Biological Alterations Induced by PRAS40-Knockout in Head and Neck Squamous Cell Carcinoma.

Authors - Gang Chen
  • KO Validated
View Article