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  • Featured Product
  • KD/KO Validated

PRC1 Polyclonal antibody

PRC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10110-2-AP

Synonyms

ASE1, PRC1

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using PRC1 Polyclonal antibody (10110-2-AP)

    WB analysis of MCF-7 using 10110-2-AP

    WB result of PRC1 antibody (10110-2-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells.

  • Western Blot (WB) analysis of various lysates using PRC1 Polyclonal antibody (10110-2-AP)

    WB analysis using 10110-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10110-2-AP (PRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRC1 Polyclonal antibody (10110-2-AP)

    IHC staining of human colon cancer using 10110-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10110-2-AP (PRC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRC1 Polyclonal antibody (10110-2-AP)

    IHC staining of human colon cancer using 10110-2-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10110-2-AP (PRC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inMCF-7 cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10110-2-AP targets PRC1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRC1 fusion protein Ag0163 相同性解析による交差性が予測される生物種
Full Name protein regulator of cytokinesis 1
Calculated molecular weight 67 kDa
Observed molecular weight 67 kDa
GenBank accession numberBC005140
Gene symbol PRC1
Gene ID (NCBI) 9055
RRIDAB_2252888
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRC1 is involved in cytokinesis. PRC1 is at high level during S and G2/M and drop dramatically after cell exit mitosis and enter G1. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a good substrate for several cyclin-dependent kinases (CDKs) in vitro and is phosphorylated in vivo at sites that are phosphorylated by CDK in vitro, suggesting that PRC1 is an in vivo CDK substrate.

Protocols

Product Specific Protocols
WB protocol for PRC1 antibody 10110-2-APDownload protocol
IHC protocol for PRC1 antibody 10110-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Immun Inflamm Dis

E2F3 renders an immunosuppressive tumor microenvironment in nasopharyngeal carcinoma: Involvements of the transcription activation of PRC1 and BIRC5

Authors - Qiang Wang
View Article