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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRC1 Recombinant antibody (84041-2-RR)

    WB analysis using 84041-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84041-2-RR (PRC1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRC1 Recombinant antibody (84041-2-RR)

    IF Staining of HepG2 using 84041-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRC1 Recombinant antibody (84041-2-RR)

    IF Staining of HepG2 using 84041-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRC1 Recombinant antibody (84041-2-RR)

    IF Staining of HepG2 using 84041-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRC1 Recombinant antibody (84041-2-RR)

    IF Staining of HepG2 using 84041-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRC1 Recombinant antibody (84041-2-RR)

    IF Staining of HepG2 using 84041-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRC1 Recombinant antibody (84041-2-RR)

    IF Staining of HepG2 using 84041-2-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (84041-2-RR, Clone: 241216H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Affinity and Kinetic Characterization of 84041-2-RR

    Affinity and Kinetic Characterization of 84041-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84041-2-RR against Human PRC1 were performed. The affinity constant is below 1 pM.

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PRC1 Recombinant antibody

PRC1 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84041-2-RR
Clone No.241216H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

ASE1, 241216H2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inMCF-7 cells, L02 cells, HeLa cells, HEK-293 cells, HCT 116 cells, C6 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84041-2-RR targets PRC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRC1 fusion protein Ag8028 相同性解析による交差性が予測される生物種
Full Name protein regulator of cytokinesis 1
Calculated molecular weight 72 kDa
GenBank accession numberBC003138
Gene Symbol PRC1
Gene ID (NCBI) 9055
RRIDAB_3671608
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43663
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively.

Protocols

Product Specific Protocols
WB protocol for PRC1 antibody 84041-2-RRDownload protocol
IF protocol for PRC1 antibody 84041-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols