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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PRCC Polyclonal antibody (12019-1-AP)

    WB analysis of HeLa using 12019-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12019-1-AP (PRCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRCC Polyclonal antibody (12019-1-AP)

    WB analysis of HeLa using 12019-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12019-1-AP (PRCC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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PRCC Polyclonal antibody

PRCC Polyclonal Antibody for WB, ELISA
Cat No. 12019-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PRCC, Proline rich protein PRCC, RCCP1, TPRC

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12019-1-AP targets PRCC in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRCC fusion protein Ag2638 相同性解析による交差性が予測される生物種
Full Name papillary renal cell carcinoma (translocation-associated)
Calculated molecular weight 491 aa, 52 kDa
Observed molecular weight 66 kDa
GenBank accession numberBC010450
Gene Symbol PRCC
Gene ID (NCBI) 5546
RRIDAB_2169667
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92733
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRCC, also named as, TPRC,regulate cell cycle progression through interaction with MAD2L2. It is frequently found in papillary renal cell carcinoma. Endogenous PRCC is coimmunoprecipitated by antibodies that recognize a variety of pre-mRNA plicing factors including SC35, PRL1 and CDC5.

Protocols

Product Specific Protocols
WB protocol for PRCC antibody 12019-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols