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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRDM10 Polyclonal antibody

PRDM10 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 23827-1-AP

Synonyms

KIAA1231, PFM7, PR domain containing 10, PRDM10, TRIS, Tristanin

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PRDM10 Polyclonal antibody (23827-1-AP)

    WB analysis of HEK-293 using 23827-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23827-1-AP (PRDM10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using PRDM10 Polyclonal antibody (23827-1-AP)

    IP experiment of mouse testis using 23827-1-AP

    IP result of anti-PRDM10 (IP:23827-1-AP, 5ug; Detection:23827-1-AP 1:500) with mouse testis tissue lysate 8000ug.

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Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23827-1-AP is PRDM10 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDM10 fusion protein Ag20605 相同性解析による交差性が予測される生物種
Full Name PR domain containing 10
Calculated molecular weight 1156 aa, 131 kDa
Observed molecular weight 120-150 kDa
GenBank accession numberBC112934
Gene symbol PRDM10
Gene ID (NCBI) 56980
RRIDAB_2879330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDM10 is a member of PRDM family, which contains a PR(PRDI-BF1 and RIZ homology) domain, and PRDM is also a subgroup of PR/SET transcription factor family for its PR domain is similar to the catalytic motif of the SET domain of histone methyltransferase(HMTase). Thus PRDM is suggested involve in the regulation of genome expression and chromatin remodeling. PRDM10 shares characteristics with the PR/SET faimly and clusters of zinc fingers DNA binding motifs. It's postualated PRDM10 has an essential role in regulating gene expression and tissue differentiation and a gene repressor. It may play a role in the pathogenesis of gangliosidosis.

Protocols

Product Specific Protocols
WB protocol for PRDM10 antibody 23827-1-APDownload protocol
IP protocol for PRDM10 antibody 23827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols