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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using PRDM15 Polyclonal antibody (25590-1-AP)

    WB analysis of Jurkat cells using 25590-1-AP

    WB result of PRDM15 (25590-1-AP, 1:500) with si-control and si-PRDM15 transfected Jurkat cells.

  • Western Blot (WB) analysis of MCF-7 cells using PRDM15 Polyclonal antibody (25590-1-AP)

    WB analysis of MCF-7 using 25590-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25590-1-AP (PRDM15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using PRDM15 Polyclonal antibody (25590-1-AP)

    IP experiment of MCF-7 using 25590-1-AP

    IP result of anti-PRDM15 (IP:25590-1-AP, 5ug; Detection:25590-1-AP 1:500) with MCF-7 cells lysate 2500ug.

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  • Featured Product
  • KD/KO Validated

PRDM15 Polyclonal antibody

PRDM15 Polyclonal Antibody for WB, IP, ELISA
Cat No. 25590-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

C21orf83, PFM15, PR domain containing 15, PRDM15, Zinc finger protein 298, ZNF298

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, Jurkat cells
Positive IP detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25590-1-AP targets PRDM15 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDM15 fusion protein Ag22296 相同性解析による交差性が予測される生物種
Full Name PR domain containing 15
Calculated molecular weight 1507 aa, 169 kDa
Observed molecular weight 169 kDa
GenBank accession numberBC067102
Gene Symbol PRDM15
Gene ID (NCBI) 63977
RRIDAB_2880145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP57071
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The PR-domain containing proteins (PRDMs) have a common involvement in the modulation of gene activities. A PR-domain family member usually produces two products, called PR-plus and PR-minus, which differ by the presence or absence of the PR domain, respectively. The PR-plus product is underexpressed or disrupted in cancer cells, whereas the PR-minus product is present or overexpressed in cancer cells. This imbalance in the amount of the two products, which is a result of either genetic or epigenetic events, appears to be a determining factor of malignancy. PRDM15 (PR domain-containing protein 15), also known as PFM15 or ZNF298, is a 1507 amino acid protein belonging to the PRDM family. Localizing to the nucleus, PRDM15 contains sixteen C2H2-type zinc fingers and one SET domain. It is believed to participate in transcriptional regulation and may be involved in cell differentiation and tumorigenesis. PRDM15 exists several isoform and molecular weight of respective isoform is 169 kDa, 135kda, 62kDa.

Protocols

Product Specific Protocols
WB protocol for PRDM15 antibody 25590-1-APDownload protocol
IP protocol for PRDM15 antibody 25590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Exp Clin Cancer Res

The RNA methyltransferase METTL16 enhances cholangiocarcinoma growth through PRDM15-mediated FGFR4 expression

Authors - Nianli Liu
  • KD Validated
View Article