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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PRDM2 Polyclonal antibody (27718-1-AP)

    WB analysis of HeLa using 27718-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27718-1-AP (PRDM2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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PRDM2 Polyclonal antibody

PRDM2 Polyclonal Antibody for WB, ELISA
Cat No. 27718-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Synonyms

GATA 3 binding protein G3B, HUMHOXY1, KMT8, Lysine N methyltransferase 8, MTB ZF, MTE binding protein, PR domain containing protein 2, PRDM2, RIZ, RIZ1, RIZ2, Zinc finger protein RIZ

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27718-1-AP targets PRDM2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDM2 fusion protein Ag26769 相同性解析による交差性が予測される生物種
Full Name PR domain containing 2, with ZNF domain
Calculated molecular weight 189 kDa
Observed molecular weight 250-280 kDa
GenBank accession numberNM_001007257
Gene Symbol PRDM2
Gene ID (NCBI) 7799
RRIDAB_2880951
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDM2, also named as KMT8, RIZ, is a 1718 amino acid protein, which belongs to the class V-like SAM-binding methyltransferase superfamily. PRDM2 localizes in the nucleus and is highly expressed in retinoblastoma cell lines and in brain tumors and is also expressed in a number of other cell lines and in brain, heart, skeletal muscle, liver and spleen. Isoform 1 is expressed in testis at much higher level than isoform 3. PRDM2 as a S-adenosyl-L-methionine-dependent histone methyltransferase that specifically methylates 'Lys-9' of histone H3. May function as a DNA-binding transcription factor. The calculated molecular weight of PRDM2 is about 189 kDa. PRDM2 exists some isoforms with 250 kDa and 280 kDa. (PMID: 26040698)

Protocols

Product Specific Protocols
WB protocol for PRDM2 antibody 27718-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols