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peroxiredoxin 2 Polyclonal antibody

Name: peroxiredoxin 2 Polyclonal antibody
Brand: Proteintech
SKU: 10545-2-AP

peroxiredoxin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10545-2-AP

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis using 10545-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10545-2-AP
WB result of peroxiredoxin 2 antibody (10545-2-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells.
IP experiment of HepG2 using 10545-2-AP
IP result of anti-peroxiredoxin 2 (IP:10545-2-AP, 4ug; Detection:10545-2-AP 1:4000) with HepG2 cells lysate 960 ug.
IHC staining of human liver using 10545-2-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 10545-2-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human hepatocirrhosis using 10545-2-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens).
IHC staining of human hepatocirrhosis using 10545-2-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens).
IF Staining of HepG2 using 10545-2-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IF Staining of HeLa using 10545-2-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 10545-2-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HepG2 cells, C2C12 cells, PC-12 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue, human hepatocirrhosis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:600-1:3000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 5 publications below
WB	See 38 publications below
IHC	See 12 publications below
IF	See 5 publications below
IP	See 3 publications below
CoIP	See 1 publications below
RIP	See 1 publications below

Product Information

10545-2-AP targets peroxiredoxin 2 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	peroxiredoxin 2 fusion protein Ag0835 相同性解析による交差性が予測される生物種
Full Name	peroxiredoxin 2
Calculated molecular weight	22 kDa
Observed molecular weight	22 kDa
GenBank accession number	BC003022
Gene symbol	peroxiredoxin 2
Gene ID (NCBI)	7001
RRID	AB_2168202
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Protocols

Product Specific Protocols
WB protocol for peroxiredoxin 2 antibody 10545-2-AP	Download protocol
IHC protocol for peroxiredoxin 2 antibody 10545-2-AP	Download protocol
IF protocol for peroxiredoxin 2 antibody 10545-2-AP	Download protocol
IP protocol for peroxiredoxin 2 antibody 10545-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. Authors - Zhiwei Liao View Article
rat	WB	Br J Pharmacol Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation. Authors - Bo Wei View Article
human	WB, IHC	Oncogene PMID: 26500057 Authors - Y-H Park View Article
human,mouse	WB,IP,CoIP	Free Radic Biol Med Oxidation resistance 1 regulates post-translational modifications of peroxiredoxin 2 in the cerebellum. Authors - Daria M Svistunova View Article
mouse	IF	Stem Cells Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells. Authors - Sun-Uk Kim View Article
human	WB,IHC	Oxid Med Cell Longev Azelaic Acid Exerts Antileukemia Effects against Acute Myeloid Leukemia by Regulating the Prdxs/ROS Signaling Pathway. Authors - Dongdong Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

peroxiredoxin 2 Polyclonal antibody

peroxiredoxin 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis using 10545-2-AP

WB analysis of HepG2 using 10545-2-AP

IP experiment of HepG2 using 10545-2-AP

IHC staining of human liver using 10545-2-AP

IHC staining of human liver using 10545-2-AP

IHC staining of human hepatocirrhosis using 10545-2-AP

IHC staining of human hepatocirrhosis using 10545-2-AP

IF Staining of HepG2 using 10545-2-AP

IF Staining of HeLa using 10545-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

PMID: 26500057

Oxidation resistance 1 regulates post-translational modifications of peroxiredoxin 2 in the cerebellum.

Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells.

Azelaic Acid Exerts Antileukemia Effects against Acute Myeloid Leukemia by Regulating the Prdxs/ROS Signaling Pathway.