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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using peroxiredoxin 2 Polyclonal antibody (20569-1-AP)

    WB analysis of HeLa using 20569-1-AP

    WB result of peroxiredoxin 2 antibody (20569-1-AP, 1:5000) with si-Control and si-peroxiredoxin 2 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using peroxiredoxin 2 Polyclonal antibody (20569-1-AP)

    WB analysis of HeLa using 20569-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20569-1-AP (peroxiredoxin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using peroxiredoxin 2 Polyclonal antibody (20569-1-AP)

    WB analysis of HeLa using 20569-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20569-1-AP (peroxiredoxin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using peroxiredoxin 2 Polyclonal antibody (20569-1-AP)

    IF Staining of HepG2 using 20569-1-AP

    Immunofluorescent analysis of HepG2 cells, using PRDX2 antibody 20569-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  • Featured Product
  • KD/KO Validated

peroxiredoxin 2 Polyclonal antibody

peroxiredoxin 2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 20569-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PRDX2, Peroxiredoxin-2, NKEF-B, NKEFB, Natural killer cell-enhancing factor B

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20569-1-AP targets peroxiredoxin 2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 2
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_005809
Gene Symbol peroxiredoxin 2
Gene ID (NCBI) 7001
RRIDAB_10695186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32119
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRDX2, also named as NKEFB, TDPX1, TSA and PRP, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. PRDX2 may play an important role in eliminating peroxides generated during metabolism. It might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The antibody is specific to PRDX2.

Protocols

Product Specific Protocols
WB protocol for peroxiredoxin 2 antibody 20569-1-APDownload protocol
IF protocol for peroxiredoxin 2 antibody 20569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols