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  • Featured Product
  • KD/KO Validated

PRDX3 Monoclonal antibody, PBS Only (Capture)

PRDX3 Monoclonal Antibody for WB, Indirect ELISA, Cytometric bead array

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

1F7F7

Cat no : 66810-1-PBS

Synonyms

Antioxidant protein 1, AOP 1, AOP1, HBC189, MER5, peroxiredoxin 3, Peroxiredoxin III, PRDX3, PRO1748, Protein MER5 homolog, Prx III, PRX3, SP 22

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRDX3 Monoclonal antibody (66810-1-Ig)

    WB analysis using 66810-1-Ig (same clone as 66810-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66810-1-Ig (PRX3 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PRDX3 Monoclonal antibody (66810-1-Ig)

    WB analysis of HEK-293 using 66810-1-Ig (same clone as 66810-1-PBS)

    WB result of PRDX3 antibody (66810-1-Ig; 1:80000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66810-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50218-2

    Cytometric bead array standard curve of MP50218-2

    Cytometric bead array standard curve of MP50218-2, PRX3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66810-1-PBS. Detection antibody: 66810-2-PBS. Standard: Ag1062. Range: 0.781-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66810-1-PBS targets PRDX3 as part of a matched antibody pair:

MP50218-2: 66810-1-PBS capture and 66810-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PRDX3 fusion protein Ag1062 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 3
Calculated molecular weight 27 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC007062
Gene symbol PRDX3
Gene ID (NCBI) 10935
RRIDAB_2882153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.