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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRDX3 Recombinant antibody (81833-1-RR)

    WB analysis using 81833-1-RR (same clone as 81833-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81833-1-RR (PRX3 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PRDX3 Recombinant antibody (81833-1-RR)

    WB analysis of HEK-293 using 81833-1-RR (same clone as 81833-1-PBS)

    WB result of PRDX3 antibody (81833-1-RR; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX3 transfected HEK-293 cells. This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PRDX3 Recombinant antibody (81833-1-RR)

    IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using PRDX3 Recombinant antibody (81833-1-RR)

    IHC staining of human lung cancer using 81833-1-RR (same clone as 81833-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 81833-1-RR (PRDX3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRDX3 Recombinant antibody (81833-1-RR)

    IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRDX3 Recombinant antibody (81833-1-RR)

    IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRDX3 Recombinant antibody (81833-1-RR)

    IF Staining of HepG2 using 81833-1-RR (same clone as 81833-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRDX3 antibody (81833-1-RR, Clone: 1L11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 81833-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81833-1-RR

    Affinity and Kinetic Characterization of 81833-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81833-1-RR against Human PRDX3 were performed. The affinity constant is below 1 pM.

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  • Featured Product
  • KD/KO Validated

PRDX3 Recombinant antibody, PBS Only

PRDX3 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81833-1-PBS
Clone No.1L11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MER5, EC:1.11.1.24, AOP-1, AOP1, Antioxidant protein 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81833-1-PBS targets PRDX3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRDX3 fusion protein Ag1062 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 3
Calculated molecular weight 27 kDa
Observed molecular weight28 kDa
GenBank accession numberBC007062
Gene Symbol PRDX3
Gene ID (NCBI) 10935
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30048
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRDX3(Peroxiredoxin 3), also named as AOP1, HBC189 and MER5, belongs to the ahpC/TSA family. It is involved in redox regulation of the cell and protects radical-sensitive enzymes from oxidative damage by a radical-generating system. PRDX3 is required for MYC-mediated proliferation, transformation, and apoptosis after glucose withdrawal and essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells (PMID:12011429). PRDX3 acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.