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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRDX6 Polyclonal antibody (13585-1-AP)

    WB analysis using 13585-1-AP (same clone as 13585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HAP1 cells using PRDX6 Polyclonal antibody (13585-1-AP)

    WB analysis of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    HAP1 (WT and PRDX6 KO) lysates prepared with RIPA buffer, 25 μg protein loaded. 13585-1-AP incubated at 1:2000 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using PRDX6 Polyclonal antibody (13585-1-AP)

    WB analysis of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    WB result of PRDX6 antibody (13585-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRDX6 transfected HeLa cells. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRDX6 Polyclonal antibody (13585-1-AP)

    WB analysis using 13585-1-AP (same clone as 13585-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13585-1-AP (PRDX6 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PRDX6 Polyclonal antibody (13585-1-AP)

    IP experiment of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    IP result of anti-PRDX6 (IP:13585-1-AP, 3ug; Detection:13585-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HAP1 cells using PRDX6 Polyclonal antibody (13585-1-AP)

    IP experiment of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    HAP1 lysates prepared and IP of peroxidoxin 6 performed using 2.0 μg of 13585-1-AP coupled to protein A- Sepharose beads. Ponceau stained transfers shown for each blot. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PRDX6 Polyclonal antibody (13585-1-AP)

    IHC staining of human brain using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PRDX6 Polyclonal antibody (13585-1-AP)

    IHC staining of human brain using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRDX6 Polyclonal antibody (13585-1-AP)

    IHC staining of human colon using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13585-1-AP (PRDX6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRDX6 Polyclonal antibody (13585-1-AP)

    IHC staining of human colon using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 13585-1-AP (PRDX6 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PRDX6 Polyclonal antibody (13585-1-AP)

    IHC staining of human brain using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using PRDX6 Polyclonal antibody (13585-1-AP)

    IHC staining of human brain using 13585-1-AP (same clone as 13585-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 13585-1-AP (PRDX6 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRDX6 Polyclonal antibody (13585-1-AP)

    IF Staining of HeLa using 13585-1-AP (same clone as 13585-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRDX6 antibody (13585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRDX6 Polyclonal antibody (13585-1-AP)

    IF Staining of HepG2 using 13585-1-AP (same clone as 13585-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed 48孔 HepG2 cells using PRDX6 antibody (13585-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HAP1 cells using PRDX6 Polyclonal antibody (13585-1-AP)

    IF Staining of HAP1 using 13585-1-AP (same clone as 13585-1-PBS)

    HAP1 WT cells (yellow outline) and PRDX6 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 13585-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 13585-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

PRDX6 Polyclonal antibody, PBS Only

PRDX6 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13585-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:2.3.1.23, EC:1.11.1.27, AOP2, Acidic calcium-independent phospholipase A2, 1-Cys PRX

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13585-1-PBS targets PRDX6 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX6 fusion protein Ag4512 相同性解析による交差性が予測される生物種
Full Name peroxiredoxin 6
Calculated molecular weight 224 aa, 25 kDa
Observed molecular weight 25-30 kDa
GenBank accession numberBC035857
Gene Symbol PRDX6
Gene ID (NCBI) 9588
RRIDAB_2168637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30041
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PRDX6 (Peroxiredoxin-6), also named as AOP2 or KIAA0106, is a unique member of the peroxiredoxin family of antioxidants. PRDX6 is highly expressed in liver and protects cells from oxidative damage by reducing H2O2 and various lipid Peroxides (PMID: 17382207). It can form a dimer(PMID:20500660).PRDX6 is expressed in all major organs, with a particularly high level in lung (PMID:15890616). Prdx6 is detected at approximately 24 to 28 kDa, and can be monosumoylated with the molecular mass of about 40 kDa (PMID: 24910119).