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KD/KO Validated

PREB Polyclonal antibody

Name: PREB Polyclonal antibody
Brand: Proteintech
SKU: 10146-2-AP

PREB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10146-2-AP

Synonyms

SEC12, Prolactin regulatory element-binding protein, Guanine nucleotide-exchange factor SEC12

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of SKOV-3 using 10146-2-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of 3T3-L1 cells using 10146-2-AP
WB result of anti-PREB (10146-2-AP, 1:250) with HepG2 and HT1080 by Dr. Dzmitry Batrakou.
WB analysis of SKOV-3 using 10146-2-AP
SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 10146-2-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10146-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10146-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of SKOV-3 using 10146-2-AP
IP result of anti-PREB (IP:10146-2-AP, 4ug; Detection:10146-2-AP 1:500) with SKOV-3 cells lysate 3200ug.
IHC staining of human prostate cancer using 10146-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse intestine using 10146-2-AP
Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 10146-2-AP (PREB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 10146-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human ovary tumor using 10146-2-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human prostate cancer using 10146-2-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HepG2 using 10146-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PREB antibody (10146-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 10146-2-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SKOV-3 cells, 3T3-L1 cells , HT-1080 cells, HepG2 cells
Positive IP detected in	SKOV-3 cells
Positive IHC detected in	mouse intestine, human ovary tumor tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 8 publications below
IF	See 1 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

10146-2-AP targets PREB in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PREB fusion protein Ag0202 相同性解析による交差性が予測される生物種
Full Name	prolactin regulatory element binding
Calculated molecular weight	45 kDa
Observed molecular weight	45 kDa
GenBank accession number	BC002765
Gene symbol	PREB
Gene ID (NCBI)	10113
RRID	AB_2168779
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.

Protocols

Product Specific Protocols
WB protocol for PREB antibody 10146-2-AP	Download protocol
IHC protocol for PREB antibody 10146-2-AP	Download protocol
IF protocol for PREB antibody 10146-2-AP	Download protocol
IP protocol for PREB antibody 10146-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	EMBO J The unfolded protein response regulates ER exit sites via SNRPB-dependent RNA splicing and contributes to bone development Authors - Muhammad Zahoor View Article
mouse,human	WB	Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis Authors - Mengyue You View Article
	IP,WB,IF	Int J Biol Macromol Cellular OCIAD2 protein is a proviral factor for hepatitis C virus replication. Authors - Zibing Yang View Article
human	WB,CoIP	J Cell Sci A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export. Authors - Jiaxin Sun KD Validated View Article
human	WB	Biochem Biophys Res Commun Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation. Authors - Yuri Urai View Article
mouse	WB	Biochim Biophys Acta Prolactin regulatory element-binding (PREB) protein regulates hepatic glucose homeostasis. Authors - Joo-Man Park View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PREB Polyclonal antibody

PREB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of SKOV-3 using 10146-2-AP

WB analysis of 3T3-L1 cells using 10146-2-AP

WB analysis of SKOV-3 using 10146-2-AP

WB analysis of HT-1080 using 10146-2-AP

WB analysis of HepG2 using 10146-2-AP

WB analysis of HepG2 using 10146-2-AP

IP experiment of SKOV-3 using 10146-2-AP

IHC staining of human prostate cancer using 10146-2-AP

IHC staining of mouse intestine using 10146-2-AP

IHC staining of human ovary tumor using 10146-2-AP

IHC staining of human ovary tumor using 10146-2-AP

IHC staining of human prostate cancer using 10146-2-AP

IF Staining of HepG2 using 10146-2-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The unfolded protein response regulates ER exit sites via SNRPB-dependent RNA splicing and contributes to bone development

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Cellular OCIAD2 protein is a proviral factor for hepatitis C virus replication.

A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.

Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.

Prolactin regulatory element-binding (PREB) protein regulates hepatic glucose homeostasis.