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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PREB Monoclonal antibody, PBS Only

PREB Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6A1

Cat no : 66981-1-PBS

Synonyms

PREB, SEC12

Validation Data Gallery

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  • Western Blot (WB) analysis of Daudi cells using PREB Monoclonal antibody (66981-1-Ig)

    WB analysis of Daudi using 66981-1-Ig (same clone as 66981-1-PBS)

    Daudi cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using PREB Monoclonal antibody (66981-1-Ig)

    WB analysis of Ramos using 66981-1-Ig (same clone as 66981-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using PREB Monoclonal antibody (66981-1-Ig)

    WB analysis of Raji using 66981-1-Ig (same clone as 66981-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66981-1-Ig (PREB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PREB Monoclonal antibody (66981-1-Ig)

    IHC staining of human prostate cancer using 66981-1-Ig (same clone as 66981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PREB Monoclonal antibody (66981-1-Ig)

    IHC staining of human prostate cancer using 66981-1-Ig (same clone as 66981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66981-1-Ig (PREB antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66981-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66981-1-PBS targets PREB in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PREB fusion protein Ag28549 相同性解析による交差性が予測される生物種
Full Name prolactin regulatory element binding
Calculated molecular weight 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC002765
Gene symbol PREB
Gene ID (NCBI) 10113
RRIDAB_2882301
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.