Validation Data Gallery
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Tested Applications
| Positive WB detected in | human lung tissue, Jurkat cells, mouse lung tissue |
| Positive IHC detected in | human ovary tumor tissue, human colon cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IHC | See 4 publications below |
| IF | See 3 publications below |
Product Information
11536-1-AP targets PREP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PREP fusion protein Ag2101 相同性解析による交差性が予測される生物種 |
| Full Name | prolyl endopeptidase |
| Calculated molecular weight | 710 aa, 81 kDa |
| Observed molecular weight | 81 kDa |
| GenBank accession number | BC030636 |
| Gene Symbol | PREP |
| Gene ID (NCBI) | 5550 |
| RRID | AB_2284249 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P48147 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PREP, also named as PE and PEP, belongs to the peptidase S9A family. It cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Together with MMPs, PREP may play an important role in the formation of PGP and thus in the pathophysiology of lung emphysema.PREP is the only enzyme directly capable of cleaving PGP from shorter portions of collagen.(PMID:21112944)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PREP antibody 11536-1-AP | Download protocol |
| IHC protocol for PREP antibody 11536-1-AP | Download protocol |
| IF protocol for PREP antibody 11536-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Gut Collagen degradation and neutrophilic infiltration: a vicious circle in inflammatory bowel disease. | ||
Oxid Med Cell Longev Increased Expression of Prolyl Endopeptidase Induced by Oxidative Stress in Nucleus Pulposus Cells Aggravates Intervertebral Disc Degeneration.
| ||
Int J Biol Macromol A novel pectin from Polygala tenuifolia blocks Aβ42 aggregation and production by enhancing insulin-degradation enzyme and neprilysin. | ||
PLoS One Cigarette smoke-induced collagen destruction; key to chronic neutrophilic airway inflammation? | ||
Am J Physiol Lung Cell Mol Physiol Cigarette smoke-induced lung emphysema in mice is associated with prolyl endopeptidase, an enzyme involved in collagen breakdown. | ||
J Gen Virol Phosphorylation of the Autographa californica multiple nucleopolyhedrovirus polyhedron envelope protein plays an important role in the formation of the occlusion body envelope. |