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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PREX2 Polyclonal antibody

PREX2 Polyclonal Antibody for IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24195-1-AP

Synonyms

P-Rex2, Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein, P REX2, DEPDC2, DEP.2

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human heart tissue using PREX2 Polyclonal antibody (24195-1-AP)

    IHC staining of human heart using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens)..

  • Immunohistochemistry (IHC) staining of human heart tissue using PREX2 Polyclonal antibody (24195-1-AP)

    IHC staining of human heart using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens)..

  • Immunohistochemistry (IHC) staining of human brain tissue using PREX2 Polyclonal antibody (24195-1-AP)

    IHC staining of human brain using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using PREX2 Polyclonal antibody (24195-1-AP)

    IHC staining of human brain using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using PREX2 Polyclonal antibody (24195-1-AP)

    IHC staining of human cerebellum using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cerebellum tissue using PREX2 Polyclonal antibody (24195-1-AP)

    IHC staining of human cerebellum using 24195-1-AP

    Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 24195-1-AP (PREX2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PREX2 Polyclonal antibody (24195-1-AP)

    IF Staining of U2OS using 24195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using 24195-1-AP (PREX2 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman heart tissue, human cerebellum tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24195-1-AP targets PREX2 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PREX2 fusion protein Ag19439 相同性解析による交差性が予測される生物種
Full Name phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
Calculated molecular weight 1606 aa, 183 kDa
Observed molecular weight 182 kDa
GenBank accession numberBC148795
Gene symbol PREX2
Gene ID (NCBI) 80243
RRIDAB_2879450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for PREX2 antibody 24195-1-APDownload protocol
IF protocol for PREX2 antibody 24195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols