PRG2 Polyclonal antibody
PRG2 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10766-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
10766-1-AP targets PRG2 in IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PRG2 fusion protein Ag1150 相同性解析による交差性が予測される生物種 |
| Full Name | proteoglycan 2, bone marrow (natural killer cell activator, eosinophil granule major basic protein) |
| Calculated molecular weight | 25 kDa |
| GenBank accession number | BC005929 |
| Gene symbol | PRG2 |
| Gene ID (NCBI) | 5553 |
| RRID | AB_2169223 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRG2, also known as BMPG, has 222 amino acid residues with a 16 N-terminal signal sequence, so the mature form has 206 amino acid residues. High levels of PRG2 are present in placenta and pregnancy serum, where it exists as a complex with several other proteins including pregnancy-associated plasma protein A (PAPPA), angiotensinogen (AGT), and C3dg. This protein can be cleaved into eosinophil granule major basic protein (MBP), which is the major constituent of the crystalline core of the eosinophil granule. MBP is highly basic and toxic to both mammalian cells and parasites, and it functions in the defense against invading parasites (PMID: 21270431).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PRG2 antibody 10766-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Allergy Immunoglobulin G4 in eosinophilic esophagitis: Immune complex formation and correlation with disease activity | ||
Exp Eye Res Role of TGF-β in tissue eosinophilia associated with vernal keratoconjunctivitis. |
