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PRICKLE1 Polyclonal antibody
PRICKLE1 Polyclonal Antibody for WB, IF-P, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF-P, FC (Intra), IP, ELISA
Conjugate
Unconjugated
Cat no : 22589-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, Neuro-2a cells |
| Positive IP detected in | mouse brain tissue |
| Positive IF-P detected in | mouse cerebellum tissue |
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 4 publications below |
Product Information
22589-1-AP targets PRICKLE1 in WB, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PRICKLE1 fusion protein Ag18407 相同性解析による交差性が予測される生物種 |
| Full Name | prickle homolog 1 (Drosophila) |
| Calculated molecular weight | 831 aa, 94 kDa |
| Observed molecular weight | 94 kDa, 55-60 kDa |
| GenBank accession number | BC114939 |
| Gene symbol | PRICKLE1 |
| Gene ID (NCBI) | 144165 |
| RRID | AB_2879129 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| IF protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| IP protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| FC protocol for PRICKLE1 antibody 22589-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex.
| ||
Development Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch. | ||
Glia The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2. | ||
Exp Neurol Mutation of the murine Prickle1 (R104Q) causes phenotypes analogous to human symptoms of epilepsy and autism. | ||
J Transl Med PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia. | ||
bioRxiv Differential Sensitivity of Midline Patterning to Mitosis during and after Primitive Streak Extension |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Murali (Verified Customer) (11-01-2022) | I will recommend this antibody for IF.
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