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  • KD/KO Validated

AMPK Alpha Polyclonal antibody

AMPK Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10929-2-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IP, ELISA

AMPK Alpha 1, PRKAA1, AMPK 1, AMPK, Acetyl-CoA carboxylase kinase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10929-2-AP targets AMPK Alpha in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, zebrafish, goat, fish, carp
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Alpha fusion protein Ag1367 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, alpha 1 catalytic subunit
Calculated molecular weight 63 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC012622
Gene Symbol AMPK Alpha 1
Gene ID (NCBI) 5562
RRIDAB_2169568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13131
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian 5-prime-AMP-activated protein kinase (AMPK) appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. PRKAA1 is also named as AMPK1, ACACA kinase, HMGCR kinase. It is a mammalian homologue of sucrose non-fermenting protein kinase (SNF-1), which belongs to a serine/threonine protein kinase family. It has 2 isoforms with molecular mass of 63-66 kDa produced by alternative splicing.10929-2-AP can recognize both AMPK Alpha 1 and AMPK Alpha 2.

Protocols

Product Specific Protocols
WB protocol for AMPK Alpha antibody 10929-2-APDownload protocol
IHC protocol for AMPK Alpha antibody 10929-2-APDownload protocol
IF protocol for AMPK Alpha antibody 10929-2-APDownload protocol
IP protocol for AMPK Alpha antibody 10929-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.

Authors - Xiao-Yi Lu
humanWB

Autophagy

Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy

Authors - Kui Wang
humanWB,IP

Autophagy

The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses.

Authors - Yongquan Hu
  • KO Validated
WB

Autophagy

A plant nonenveloped double-stranded RNA virus activates and co-opts BNIP3-mediated mitophagy to promote persistent infection in its insect vector.

Authors - Qifu Liang
humanWB

J Hazard Mater

Mitigation of honokiol on fluoride-induced mitochondrial oxidative stress, mitochondrial dysfunction, and cognitive deficits through activating AMPK/PGC-1α/Sirt3.

Authors - Dongmei Wang
humanWB

J Hazard Mater

Graphene oxide disrupted mitochondrial homeostasis through inducing intracellular redox deviation and autophagy-lysosomal network dysfunction in SH-SY5Y cells.

Authors - Feng Xiaoli
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