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KD/KO Validated

AMPK Alpha 2 Polyclonal antibody

Name: AMPK Alpha 2 Polyclonal antibody
Brand: Proteintech
SKU: 18167-1-AP

AMPK Alpha 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 18167-1-AP

Synonyms

AMPK, AMPK alpha 2, AMPK subunit alpha 2, AMPK2, AMPKA2, AMPKα2, PRKAA, PRKAA2

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 18167-1-AP
WB result of AMPK alpha 2 antibody (18167-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMPK alpha 2 transfected HEK-293 cells.
WB analysis using 18167-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18167-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 18167-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 18167-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat ovary using 18167-1-AP
rat ovary tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 18167-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse skeletal muscle using 18167-1-AP
IP result of anti-AMPK Alpha 2 (IP:18167-1-AP, 4ug; Detection:18167-1-AP 1:1000) with mouse skeletal muscle tissue lysate 3600ug.
IHC staining of human breast cancer using 18167-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 18167-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18167-1-AP (AMPK alpha 2 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 18167-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 18167-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 18167-1-AP (AMPK alpha 2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of MCF-7 using 18167-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using AMPK Alpha 2 antibody (18167-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 18167-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells, human skeletal muscle tissue, rat ovary tissue, SKOV-3 cells, MCF-7 cells
Positive IP detected in	mouse skeletal muscle tissue
Positive IHC detected in	human breast cancer tissue, mouse skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 9 publications below
WB	See 47 publications below
IHC	See 4 publications below
IF	See 4 publications below
IP	See 3 publications below

Product Information

The immunogen of 18167-1-AP is AMPK Alpha 2 Fusion Protein expressed in E. coli.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AMPK Alpha 2 fusion protein Ag12796 相同性解析による交差性が予測される生物種
Full Name	protein kinase, AMP-activated, alpha 2 catalytic subunit
Calculated molecular weight	552 aa, 62 kDa
Observed molecular weight	62 kDa
GenBank accession number	BC069680
Gene symbol	AMPK Alpha 2
Gene ID (NCBI)	5563
RRID	AB_10695046
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRKAA2(protein kinase, AMP-activated, alpha 2 catalytic subunit), also named as AMPKA2, AMPK, PRKAA, AMPK2, belongs to the CAMK Ser/Thr protein kinase family and SNF1 subfamily. PRKAA2 is an αβγ heterotrimer that is activated by low cellular energy status, such as decreases in both the ATP/AMP ratio and the phosphocreatine content and it is a glycogen synthase kinase, phosphorylating Ser7 at the NH2 terminus, which decreases glycogen synthase activity (PMID:14532170). The protein can be ubiquitinated (PMID:21224036).

Protocols

Product Specific Protocols
WB protocol for AMPK Alpha 2 antibody 18167-1-AP	Download protocol
IHC protocol for AMPK Alpha 2 antibody 18167-1-AP	Download protocol
IF protocol for AMPK Alpha 2 antibody 18167-1-AP	Download protocol
IP protocol for AMPK Alpha 2 antibody 18167-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell Metab Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism. Authors - Yi Fu View Article
human	WB	Dev Cell Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation Authors - Bin Tan View Article
rat	WB	ACS Appl Mater Interfaces Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair. Authors - Lin Huang View Article
human	WB	Autophagy TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer. Authors - Cefan Zhou View Article
rat	WB	Aging Cell Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway. Authors - Juan Ji View Article
human,mouse	WB,IHC,IF,IP	Cell Mol Gastroenterol Hepatol Targeting USP9X-AMPK Axis in ARID1A-Deficient Hepatocellular Carcinoma. Authors - Feng-Kun Zhang KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPK Alpha 2 Polyclonal antibody

AMPK Alpha 2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of HEK-293 using 18167-1-AP

WB analysis using 18167-1-AP

WB analysis of HEK-293 using 18167-1-AP

WB analysis using 18167-1-AP

WB analysis of HEK-293 using 18167-1-AP

WB analysis of rat ovary using 18167-1-AP

WB analysis of human skeletal muscle using 18167-1-AP

IP experiment of mouse skeletal muscle using 18167-1-AP

IHC staining of human breast cancer using 18167-1-AP

IHC staining of human breast cancer using 18167-1-AP

IHC staining of mouse skeletal muscle using 18167-1-AP

IHC staining of mouse skeletal muscle using 18167-1-AP

IF Staining of MCF-7 using 18167-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism.

Endothelial progenitor cells control remodeling of uterine spiral arteries for the establishment of utero-placental circulation

Biocompatible Chitin Hydrogel Incorporated with PEDOT Nanoparticles for Peripheral Nerve Repair.

TSPAN1 promotes autophagy flux and mediates cooperation between WNT-CTNNB1 signaling and autophagy via the MIR454-FAM83A-TSPAN1 axis in pancreatic cancer.

Antagonizing peroxisome proliferator-activated receptor γ facilitates M1-to-M2 shift of microglia by enhancing autophagy via the LKB1-AMPK signaling pathway.

Targeting USP9X-AMPK Axis in ARID1A-Deficient Hepatocellular Carcinoma.