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KD/KO Validated

AMPK Beta 1 Polyclonal antibody

Name: AMPK Beta 1 Polyclonal antibody
Brand: Proteintech
SKU: 10308-1-AP

AMPK Beta 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 10308-1-AP

Synonyms

PRKAB1, AMPKb, AMPK β, AMPK subunit beta-1, AMPK subunit beta 1

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of mouse liver using 10308-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10308-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10308-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 10308-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10308-1-AP (AMPK beta 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 10308-1-AP
IP result of anti-AMPK Beta 1 (IP:10308-1-AP, 4ug; Detection:10308-1-AP 1:5000) with mouse liver tissue lysate 1960 ug.
IHC staining of human lung cancer using 10308-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10308-1-AP (AMPK beta 1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10308-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10308-1-AP (AMPK beta 1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10308-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AMPK Beta 1 antibody (10308-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Cat No. 10308-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, A431 cells, HEK-293 cells, HeLa cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 12 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

10308-1-AP targets AMPK Beta 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	AMPK Beta 1 fusion protein Ag0301 相同性解析による交差性が予測される生物種
Full Name	protein kinase, AMP-activated, beta 1 non-catalytic subunit
Calculated molecular weight	38 kDa
Observed molecular weight	38 kDa
GenBank accession number	BC001007
Gene symbol	PRKAB1
Gene ID (NCBI)	5564
RRID	AB_513239
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

AMPK Beta 1 (5'-AMP-activated protein kinase subunit beta-1) is also named as PRKAB1 and AMPK. AMPK, a serine/threonine kinase that exists as a heterotrimer comprised of a catalytic α-subunit and regulatory β- and γ-subunits, has been recognized as a sensor of cellular energy homeostasis (PMID: 21937710). AMPK regulates key metabolic enzymes, cell growth, apoptosis, gene transcription, and protein synthesis (PMID: 12829246). AMPK is an energy sensor and plays an essential role in the control of cellular bioenergetics by responding to various stresses including those that induce changes in the cellular AMP:ATP ratio or modulation in intracellular calcium (PMID: 27812976, PMID: 26616193). Recent studies have shown that AMPK mediates the inhibition of cell proliferation and growth of tumor cells (PMID: 16613876). AMPK also inhibits the expression of Glut1 and glycolysis in Tregs by inhibiting mTORC1 signaling (PMID: 25477880).

Protocols

Product Specific Protocols
WB protocol for AMPK Beta 1 antibody 10308-1-AP	Download protocol
IHC protocol for AMPK Beta 1 antibody 10308-1-AP	Download protocol
IF protocol for AMPK Beta 1 antibody 10308-1-AP	Download protocol
IP protocol for AMPK Beta 1 antibody 10308-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP	Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress. Authors - Qidong Li KD Validated View Article
mouse	WB	Acta Pharm Sin B Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy Authors - Chang Xiao View Article
mouse	WB,IF	Theranostics Therapeutic inhibition of miR-802 protects against obesity through AMPK-mediated regulation of hepatic lipid metabolism. Authors - Yangyue Ni View Article
human,mouse	WB	J Ethnopharmacol Mulberry leaves attenuate D-galactose-induced aging in vivo and in vitro Authors - Yan Zhu View Article
human	WB	Hum Cell RNF126 contributes to stem cell-like properties and metastasis in hepatocellular carcinoma through ubiquitination and degradation of LKB1 Authors - Jie Huang View Article
rat	WB	Dig Dis Sci Heme Oxygenase-1-Modified BMMSCs Activate AMPK-Nrf2-FTH1 to Reduce Severe Steatotic Liver Ischemia-Reperfusion Injury Authors - Xiaorong Tian View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPK Beta 1 Polyclonal antibody

AMPK Beta 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of mouse liver using 10308-1-AP

WB analysis of HEK-293 using 10308-1-AP

WB analysis of HeLa using 10308-1-AP

WB analysis of A431 using 10308-1-AP

IP experiment of mouse liver using 10308-1-AP

IHC staining of human lung cancer using 10308-1-AP

IHC staining of human lung cancer using 10308-1-AP

IF Staining of HeLa using 10308-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.

Engineering cannabidiol synergistic carbon monoxide nanocomplexes to enhance cancer therapy via excessive autophagy

Therapeutic inhibition of miR-802 protects against obesity through AMPK-mediated regulation of hepatic lipid metabolism.

Mulberry leaves attenuate D-galactose-induced aging in vivo and in vitro

RNF126 contributes to stem cell-like properties and metastasis in hepatocellular carcinoma through ubiquitination and degradation of LKB1

Heme Oxygenase-1-Modified BMMSCs Activate AMPK-Nrf2-FTH1 to Reduce Severe Steatotic Liver Ischemia-Reperfusion Injury