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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

AMPK Beta 2 Polyclonal antibody

AMPK Beta 2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14429-1-AP

Synonyms

PRKAB2, AMPK subunit beta-2, AMPK subunit beta 2, AMPK, 5'-AMP-activated protein kinase subunit beta-2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    WB analysis using 14429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of fetal human brain tissue using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    WB analysis of fetal human brain using 14429-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    WB analysis of human heart using 14429-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    WB analysis of human brain using 14429-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14429-1-AP (AMPK beta 2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    IHC staining of human colon using 14429-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    IHC staining of human colon using 14429-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    IHC staining of human kidney using 14429-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    IHC staining of human kidney using 14429-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14429-1-AP (AMPK Beta 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AMPK Beta 2 Polyclonal antibody (14429-1-AP)

    IF Staining of HeLa using 14429-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AMPK Beta 2 antibody (14429-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, human heart tissue, human brain tissue, fetal human brain tissue, mouse testis tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman colon tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14429-1-AP targets AMPK Beta 2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AMPK Beta 2 fusion protein Ag5806 相同性解析による交差性が予測される生物種
Full Name protein kinase, AMP-activated, beta 2 non-catalytic subunit
Calculated molecular weight 30 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC053610
Gene symbol AMPK Beta 2
Gene ID (NCBI) 5565
RRIDAB_2252925
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
IHC protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
IF protocol for AMPK Beta 2 antibody 14429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols