PRKACB Polyclonal antibody

Name: PRKACB Polyclonal antibody
Brand: Proteintech
SKU: 12232-1-AP

PRKACB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 12232-1-AP

Synonyms

PKACB, PKA C-beta, PKA C beta, EC:2.7.11.11

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICC

WB analysis of MCF-7 using 12232-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 12232-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 12232-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 12232-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 12232-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 12232-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12232-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 12232-1-AP
IP result of anti-PRKACB (IP:12232-1-AP, 4ug; Detection:12232-1-AP 1:500) with mouse brain tissue lysate 2640ug.
IHC staining of mouse brain using 12232-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 12232-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human medulloblastoma using 12232-1-AP
Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12232-1-AP (PRKACB antibody) at dilution of 1:50 (under 10x lens).
IF Staining of HeLa using 12232-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKACB antibody (12232-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L).

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Cat No. 12232-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, A549 cells, HT-1080 cells, mouse brain tissue, mouse heart tissue, PC-3 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse brain tissue, human medulloblastoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

12232-1-AP targets PRKACB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRKACB fusion protein Ag2870 相同性解析による交差性が予測される生物種
Full Name	protein kinase, cAMP-dependent, catalytic, beta
Calculated molecular weight	41 kDa, 46 kDa
Observed molecular weight	36-55 kDa
GenBank accession number	BC016285
Gene symbol	PRKACB
Gene ID (NCBI)	5567
RRID	AB_2170177
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.

Protocols

Product Specific Protocols
WB protocol for PRKACB antibody 12232-1-AP	Download protocol
IHC protocol for PRKACB antibody 12232-1-AP	Download protocol
IF protocol for PRKACB antibody 12232-1-AP	Download protocol
IP protocol for PRKACB antibody 12232-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Int J Nanomedicine Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway. Authors - Yang Fu View Article
mouse,rat	WB	Biomed Pharmacother Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway. Authors - Yang Fu View Article
human	WB	Biomed Res Int MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB. Authors - Yongxia Wang View Article
human	WB	Int J Mol Sci Dynamic Alteration Profile and New Role of RNA m6A Methylation in Replicative and H2O2-Induced Premature Senescence of Human Embryonic Lung Fibroblasts Authors - Fan Wu View Article
human	IHC	Front Med (Lausanne) A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma Authors - Fengyan Wang View Article
mouse	WB,IF,IP	CNS Neurosci Ther Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation Authors - Limin Ma View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKACB Polyclonal antibody

PRKACB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Validation Data Gallery

WB analysis of MCF-7 using 12232-1-AP

WB analysis of mouse heart using 12232-1-AP

WB analysis of mouse brain using 12232-1-AP

WB analysis of MCF-7 using 12232-1-AP

WB analysis of HT-1080 using 12232-1-AP

WB analysis of A549 using 12232-1-AP

WB analysis of PC-3 using 12232-1-AP

IP experiment of mouse brain using 12232-1-AP

IHC staining of mouse brain using 12232-1-AP

IHC staining of mouse brain using 12232-1-AP

IHC staining of human medulloblastoma using 12232-1-AP

IF Staining of HeLa using 12232-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway.

Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.

MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB.

Dynamic Alteration Profile and New Role of RNA m6A Methylation in Replicative and H2O2-Induced Premature Senescence of Human Embryonic Lung Fibroblasts

A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma

Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation