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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKAR1A Polyclonal antibody

PRKAR1A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20358-1-AP

Synonyms

PPNAD1, PKR1, CNC1, CNC, CAR

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKAR1A Polyclonal antibody (20358-1-AP)

    WB analysis using 20358-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRKAR1A Polyclonal antibody (20358-1-AP)

    WB analysis of HeLa using 20358-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20358-1-AP (PRKAR1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRKAR1A Polyclonal antibody (20358-1-AP)

    IHC staining of mouse testis using 20358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PRKAR1A Polyclonal antibody (20358-1-AP)

    IHC staining of human stomach cancer using 20358-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PRKAR1A Polyclonal antibody (20358-1-AP)

    IHC staining of human stomach cancer using 20358-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat salivary gland tissue using PRKAR1A Polyclonal antibody (20358-1-AP)

    IHC staining of rat salivary gland using 20358-1-AP

    Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat salivary gland tissue using PRKAR1A Polyclonal antibody (20358-1-AP)

    IHC staining of rat salivary gland using 20358-1-AP

    Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRKAR1A Polyclonal antibody (20358-1-AP)

    IHC staining of mouse brain using 20358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRKAR1A Polyclonal antibody (20358-1-AP)

    IHC staining of mouse brain using 20358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20358-1-AP (PRKAR1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKAR1A Polyclonal antibody (20358-1-AP)

    IF Staining of HeLa using 20358-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKAR1A antibody (20358-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKAR1A Polyclonal antibody (20358-1-AP)

    IF Staining of HeLa using 20358-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20358-1-AP (PRKAR1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse testis tissue, human stomach cancer tissue, mouse brain tissue, rat salivary gland tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20358-1-AP targets PRKAR1A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKAR1A fusion protein Ag14216 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, regulatory, type I, alpha (tissue specific extinguisher 1)
Calculated molecular weight 381 aa, 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberBC036285
Gene symbol PRKAR1A
Gene ID (NCBI) 5573
RRIDAB_10697659
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PKA is a heterotetramer inactive kinase composed of two regulatory and two catalytic submits. The regulatory subunits are encoded by four genes (PRKAR1A, PRKAR2A, PRKAR1B, and PRKAR2B). PRKAR1A, a gene coding for the cAMP-dependent protein kinase (PKA) 1α regulatory submit, is located on human chromosome 17q22-24. PRKAR1A protein insufficiency and PKA dysregulation have been implicated in various types of disorders, including Albright hereditary osteodystrophy (AHO), pseudohypoparathyroidism (PHP), acrodysostosis (ACRDYS), and Carney complex. PRKAR1A protein expression level was significantly dysregulated in multiple primary carcinomas and distant metastases, such as cardiac myxomas, odontogenic myxomas, anaplastic thyroid carcinomas, breast cancer, pediatric pituitary adenomas, and Schwann cell tumors.

Protocols

Product Specific Protocols
WB protocol for PRKAR1A antibody 20358-1-APDownload protocol
IHC protocol for PRKAR1A antibody 20358-1-APDownload protocol
IF protocol for PRKAR1A antibody 20358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Systematically defining selective autophagy receptor-specific cargo using autophagosome content profiling.

Authors - Susanne Zellner
View Article