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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKAR2A Monoclonal antibody, PBS Only

PRKAR2A Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2B3

Cat no : 67751-1-PBS

Synonyms

PKR2, PRKAR2, PRKAR2A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKAR2A Monoclonal antibody (67751-1-Ig)

    WB analysis using 67751-1-Ig (same clone as 67751-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67751-1-Ig (PRKAR2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRKAR2A Monoclonal antibody (67751-1-Ig)

    IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRKAR2A Monoclonal antibody (67751-1-Ig)

    IHC staining of human colon cancer using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using PRKAR2A Monoclonal antibody (67751-1-Ig)

    IHC staining of mouse colon using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using PRKAR2A Monoclonal antibody (67751-1-Ig)

    IHC staining of mouse colon using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 67751-1-Ig (PRKAR2A antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKAR2A Monoclonal antibody (67751-1-Ig)

    IF Staining of HeLa using 67751-1-Ig (same clone as 67751-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PRKAR2A antibody (67751-1-Ig, Clone: 1B2B3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67751-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67751-1-PBS targets PRKAR2A in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRKAR2A fusion protein Ag29893 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, regulatory, type II, alpha
Calculated molecular weight 43 kDa
Observed molecular weight50 kDa
GenBank accession numberBC002763
Gene symbol PRKAR2A
Gene ID (NCBI) 5576
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The effects of cAMP in most tissues and cell types are mainly modulated via protein kinase A, a heterotetrameric protein complex consisting of two regulatory and two catalytic subunits. The regulatory subunit of cAMP-dependent protein kinase (PRKAR2A) is one of the regulatory subunits and the gene is located on chromosome region 3p21.3-p21.2. The expression of PRKAR2A is tightly regulated during spermatogenesis, a significant increase in expression of this gene was also found in the human myometrium during pregnancy.