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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKAR2B Polyclonal antibody

PRKAR2B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, zebrafish

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20845-1-AP

Synonyms

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKAR2B Polyclonal antibody (20845-1-AP)

    WB analysis using 20845-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20845-1-AP (PRKAR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PRKAR2B Polyclonal antibody (20845-1-AP)

    IHC staining of human stomach cancer using 20845-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 20845-1-AP (PRKAR2B antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PRKAR2B Polyclonal antibody (20845-1-AP)

    IHC staining of human ovary using 20845-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20845-1-AP (PRKAR2B antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PRKAR2B Polyclonal antibody (20845-1-AP)

    IHC staining of human ovary using 20845-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 20845-1-AP (PRKAR2B antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20845-1-AP targets PRKAR2B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, zebrafish samples.

Tested Reactivity human, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKAR2B fusion protein Ag11866 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, regulatory, type II, beta
Calculated molecular weight 418 aa, 46 kDa
GenBank accession numberBC075800
Gene symbol PRKAR2B
Gene ID (NCBI) 5577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRKAR2B antibody 20845-1-APDownload protocol
IHC protocol for PRKAR2B antibody 20845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols