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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKAR2B Polyclonal antibody

PRKAR2B Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 28351-1-AP

Synonyms

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKAR2B Polyclonal antibody (28351-1-AP)

    WB analysis using 28351-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRKAR2B Polyclonal antibody (28351-1-AP)

    WB analysis using 28351-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PRKAR2B Polyclonal antibody (28351-1-AP)

    IP experiment of mouse brain using 28351-1-AP

    IP result of anti-PRKAR2B (IP:28351-1-AP, 4ug; Detection:28351-1-AP 1:1000) with mouse brain tissue lysate 1080 ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PRKAR2B Polyclonal antibody (28351-1-AP)

    IHC staining of human stomach cancer using 28351-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PRKAR2B Polyclonal antibody (28351-1-AP)

    IF Staining of Neuro-2a using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of Neuro-2a cells using PRKAR2B Polyclonal antibody (28351-1-AP)

    IF Staining of Neuro-2a using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using PRKAR2B Polyclonal antibody (28351-1-AP)

    IF Staining of SH-SY5Y using 28351-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of SH-SY5Y cells using PRKAR2B Polyclonal antibody (28351-1-AP)

    FC experiment of SH-SY5Y using 28351-1-AP

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PRKAR2B (28351-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inY79 cells, human testis tissue, mouse brain tissue, mouse eye tissue, rat brain tissue, mouse testis tissue
Positive IP detected inmouse brain tissue
Positive IF/ICC detected inNeuro-2a cells, SH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

28351-1-AP targets PRKAR2B in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKAR2B fusion protein Ag28787 相同性解析による交差性が予測される生物種
Full Name protein kinase, cAMP-dependent, regulatory, type II, beta
Calculated molecular weight 418 aa, 46 kDa
Observed molecular weight46-50 kDa
GenBank accession numberBC075800
Gene symbol PRKAR2B
Gene ID (NCBI) 5577
RRIDAB_2918156
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRKAR2B antibody 28351-1-APDownload protocol
IHC protocol for PRKAR2B antibody 28351-1-APDownload protocol
IF protocol for PRKAR2B antibody 28351-1-APDownload protocol
IP protocol for PRKAR2B antibody 28351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Inflamm Res

Comprehensive Analysis of Fatty Acid Metabolism in Diabetic Nephropathy from the Perspective of Immune Landscapes, Diagnosis and Precise Therapy

Authors - Enyi Zhu
View Article