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  • Featured Product
  • KD/KO Validated

PKC Alpha Polyclonal antibody

PKC Alpha Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 21991-1-AP

Synonyms

PRKCA, PKCα, PKC-A, PKCA, PKC A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKC Alpha Polyclonal antibody (21991-1-AP)

    WB analysis using 21991-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PKC Alpha Polyclonal antibody (21991-1-AP)

    WB analysis using 21991-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC Alpha antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PKC Alpha Polyclonal antibody (21991-1-AP)

    WB analysis of HEK-293 using 21991-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using PKC Alpha Polyclonal antibody (21991-1-AP)

    WB analysis of Jurkat using 21991-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PKC Alpha Polyclonal antibody (21991-1-AP)

    WB analysis using 21991-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PKC Alpha Polyclonal antibody (21991-1-AP)

    WB analysis using 21991-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21991-1-AP (PKC alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of NIH/3T3 cells using PKC Alpha Polyclonal antibody (21991-1-AP)

    IP experiment of NIH/3T3 using 21991-1-AP

    IP result of anti-PKC Alpha (IP:21991-1-AP, 4ug; Detection:21991-1-AP 1:3000) with NIH/3T3 cells lysate 1320 ug.

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PKC Alpha Polyclonal antibody (21991-1-AP)

    IHC staining of human thyroid cancer using 21991-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human thyroid cancer tissue using PKC Alpha Polyclonal antibody (21991-1-AP)

    IHC staining of human thyroid cancer using 21991-1-AP

    Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21991-1-AP (PKC alpha antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PKC Alpha Polyclonal antibody (21991-1-AP)

    IHC staining of mouse brain using 21991-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21991-1-AP (PKC Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PKC Alpha Polyclonal antibody (21991-1-AP)

    IHC staining of mouse brain using 21991-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21991-1-AP (PKC Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Alpha Polyclonal antibody (21991-1-AP)

    IF Staining of HeLa using 21991-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PKC Alpha Polyclonal antibody (21991-1-AP)

    IF Staining of HepG2 using 21991-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PKC Alpha antibody (21991-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PKC Alpha Polyclonal antibody (21991-1-AP)

    IF Staining of NIH/3T3 using 21991-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using 21991-1-AP (PKC alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using PKC Alpha Polyclonal antibody (21991-1-AP)

    FC experiment of HeLa using 21991-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PKC Alpha (21991-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Jurkat cells using PKC Alpha Polyclonal antibody (21991-1-AP)

    FC experiment of Jurkat using 21991-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PKC Alpha Polyclonal antibody (21991-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, human peripheral blood platelets, rat kidney tissue
Positive IP detected inNIH/3T3 cells
Positive IHC detected inhuman thyroid cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, NIH/3T3 cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21991-1-AP targets PKC Alpha in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Alpha fusion protein Ag17275 相同性解析による交差性が予測される生物種
Full Name protein kinase C, alpha
Calculated molecular weight 77 kDa
Observed molecular weight 77 kDa
GenBank accession numberAK055431
Gene symbol PKC Alpha
Gene ID (NCBI) 5578
RRIDAB_2878965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Protocols

Product Specific Protocols
WB protocol for PKC Alpha antibody 21991-1-APDownload protocol
IHC protocol for PKC Alpha antibody 21991-1-APDownload protocol
IF protocol for PKC Alpha antibody 21991-1-APDownload protocol
IP protocol for PKC Alpha antibody 21991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

MED13L integrates Mediator-regulated epigenetic control into lung cancer radiosensitivity.

Authors - Nasha Zhang
View Article
humanIF

Cell Death Dis

ATF4/CEMIP/PKCα promotes anoikis resistance by enhancing protective autophagy in prostate cancer cells.

Authors - Ying Yu
View Article
humanWB

Cell Death Differ

β-Trcp ubiquitin ligase and RSK2 kinase-mediated degradation of FOXN2 promotes tumorigenesis and radioresistance in lung cancer.

Authors - Jia Ma
View Article
humanWB

Oncogene

β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma.

Authors - Yanwei Lu
View Article
humanWB

Oncogenesis

Blockage of Orai1-Nucleolin interaction meditated calcium influx attenuates breast cancer cells growth

Authors - Chunming Gu
View Article
humanWB

Front Pharmacol

Cellular mechanism of action of forsythiaside for the treatment of diabetic kidney disease

Authors - Chunmei Xu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Alessandro (Verified Customer) (11-07-2022)

no unspecific staining, great outcome

  • Applications: Immunofluorescence
FH

Chiara (Verified Customer) (10-04-2021)

Works very well in Immunoblot

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: mesenchymal Stem cell
FH

Samantha (Verified Customer) (02-24-2020)

Bovine vascular smooth muscle cells were transfected with either control siRNA or PKCalpha siRNA for up to 7 days, with transfections repeated every 48-72 hours. Blocking agent: 5% (w/v) milk in TBST for 1hrPrimary antibody diluted in 5% (w/v) milk in TBST; incubated overnight at 4 degrees.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:750
  • Cell Tissue Type: Bovine vascular smooth muscle cells
PKC Alpha Antibody Western Blot, validation (1:750 dilution) in Bovine vascular smooth muscle cells (Cat no:21991-1-AP)
FH

Ryan (Verified Customer) (01-11-2018)

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Zebrafish Retina
PKC Alpha Antibody Immunohistochemistry, validation (1:100 dilution) in Zebrafish Retina (Cat no:21991-1-AP)