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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PKC Alpha Recombinant antibody

PKC Alpha Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240926E1

Cat no : 83840-6-RR

Synonyms

PRKCA, PKC-A, PKCA, PKC A, EC:2.7.11.13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PKC Alpha Recombinant antibody (83840-6-RR)

    WB analysis using 83840-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83840-6-RR (PKC Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PKC Alpha Recombinant antibody (83840-6-RR)

    IHC staining of human stomach cancer using 83840-6-RR

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83840-6-RR (PKC Alpha antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PKC Alpha Recombinant antibody (83840-6-RR)

    IF Staining of HeLa using 83840-6-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKCA antibody (83840-6-RR, Clone: 240926E1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using PKC Alpha Recombinant antibody (83840-6-RR)

    FC experiment of HepG2 using 83840-6-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PRKCA Recombinant antibody (83840-6-RR, Clone:240926E1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83840-6-RR

    Affinity and Kinetic Characterization of 83840-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83840-6-RR against Human PRKCA were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells, C6 cells, human platelets tissue, rat brain tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83840-6-RR is PKC Alpha Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PKC Alpha fusion protein Ag1025 相同性解析による交差性が予測される生物種
Full Name protein kinase C, alpha
Observed molecular weight77 kDa
GenBank accession numberBC109274
Gene symbol PKC Alpha
Gene ID (NCBI) 5578
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PRKCA is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

Protocols

Product Specific Protocols
WB protocol for PKC Alpha antibody 83840-6-RRDownload protocol
IHC protocol for PKC Alpha antibody 83840-6-RRDownload protocol
IF protocol for PKC Alpha antibody 83840-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols