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  • KD/KO Validated

PKC Delta Polyclonal antibody

PKC Delta Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 19132-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

PRKCD, PKCD, nPKC-delta, MAY1, EC:2.7.11.13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inA431 cells, Jurkat cells, MCF-7 cells, HT-1080 cells, mouse colon tissue, HUVEC cells
Positive IP detected inA431 cells
Positive IHC detected inhuman brain tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells, U-251 cells, HeLa cells, A431 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19132-1-AP targets PKC Delta in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Delta fusion protein Ag5631 相同性解析による交差性が予測される生物種
Full Name protein kinase C, delta
Calculated molecular weight 78 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC043350
Gene Symbol PKC Delta
Gene ID (NCBI) 5580
RRIDAB_10637263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05655
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Delta antibody 19132-1-APDownload protocol
IHC protocol for PKC Delta antibody 19132-1-APDownload protocol
IF protocol for PKC Delta antibody 19132-1-APDownload protocol
IP protocol for PKC Delta antibody 19132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF,IP,CoIP

Oncogene

TRIM69 suppressed the anoikis resistance and metastasis of gastric cancer through ubiquitin‒proteasome-mediated degradation of PRKCD

Authors - Linqing Sun
mouseWB

Virol Sin

Rottlerin plays an antiviral role at early and late steps of Zika virus infection

Authors - Shili Zhou
  • KD Validated
ratWB

Food Chem Toxicol

G-Rh4 improves pancreatic β-cells dysfunction in vivo and in vitro by increased expression of Nrf2 and its target genes.

Authors - Yao Liu
WB

Fish Shellfish Immunol

In vivo study of a novel protein kinase C that mediates immunocompetence and catecholamine biosynthesis in hemocytes of Litopenaeus vannamei by using its potential competitive inhibitor, bisindolylmaleimide I.

Authors - Zhong-Wen Chang
mouseWB

iScience

Induction of Inflammatory Macrophages in the Gut and Extra-Gut Tissues by Colitis-Mediated Escherichia coli.

Authors - Houbao Qi
mouseWB

Neurochem Int

Protein kinase C-mediated impairment of glutamine outward transport and SN1 transporter distribution by ammonia in mouse cortical astrocytes.

Authors - Katarzyna Dąbrowska
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