• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKCQ-Specific Polyclonal antibody

PRKCQ-Specific Polyclonal Antibody for WB, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 19982-1-AP

Synonyms

PRKCQ, Protein kinase C theta type, protein kinase C, PRKCT, nPKC-theta

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PRKCQ-Specific Polyclonal antibody (19982-1-AP)

    WB analysis using 19982-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRKCQ-Specific Polyclonal antibody (19982-1-AP)

    WB analysis using 19982-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19982-1-AP (PRKCQ-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using PRKCQ-Specific Polyclonal antibody (19982-1-AP)

    IP experiment of K-562 using 19982-1-AP

    IP result of anti-PRKCQ-Specific (IP:19982-1-AP, 4ug; Detection:19982-1-AP 1:500) with K-562 cells lysate 1600 ug.

  • Flow cytometry (FC) experiment of K-562 cells using PRKCQ-Specific Polyclonal antibody (19982-1-AP)

    FC experiment of K-562 using 19982-1-AP

    1x10^6 K-562 cells were intracellularly stained with 0.2 ug PRKCQ-Specific Polyclonal antibody (19982-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IP detected inK-562 cells
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19982-1-AP targets PRKCQ-Specific in WB, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein kinase C, theta
Calculated molecular weight 82 kDa
Observed molecular weight 72-74 kDa
GenBank accession numberNM_006257
Gene symbol PRKCQ
Gene ID (NCBI) 5588
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKCQ, also named as PRKCT and nPKC-theta, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and PKC subfamily. PRKCQ catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. PRKCQ is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. It is essential for T-cell receptor (TCR)-mediated T-cell activation, but it is dispensable during TCR-dependent thymocyte development. PRKCQ links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. It is required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. This antibody is specific to PRKCQ.

Protocols

Product Specific Protocols
WB protocol for PRKCQ-Specific antibody 19982-1-APDownload protocol
IP protocol for PRKCQ-Specific antibody 19982-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols