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  • Featured Product
  • KD/KO Validated

PRKCSH Polyclonal antibody, PBS Only

PRKCSH Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12148-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKCSH Polyclonal antibody (12148-1-AP)

    WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRKCSH Polyclonal antibody (12148-1-AP)

    WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PRKCSH Polyclonal antibody (12148-1-AP)

    IP experiment of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    IP result of anti-PRKCSH (IP:12148-1-AP, 4ug; Detection:12148-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRKCSH Polyclonal antibody (12148-1-AP)

    IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRKCSH Polyclonal antibody (12148-1-AP)

    IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRKCSH Polyclonal antibody (12148-1-AP)

    IHC staining of human colon using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKCSH Polyclonal antibody (12148-1-AP)

    IF Staining of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12148-1-AP (PRKCSH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12148-1-PBS targets PRKCSH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKCSH fusion protein Ag2796 相同性解析による交差性が予測される生物種
Full Name protein kinase C substrate 80K-H
Calculated molecular weight 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC013586
Gene symbol PRKCSH
Gene ID (NCBI) 5589
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.