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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKCSH Polyclonal antibody (12148-1-AP)

    WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PRKCSH Polyclonal antibody (12148-1-AP)

    WB analysis using 12148-1-AP (same clone as 12148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12148-1-AP (PRKCSH antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using PRKCSH Polyclonal antibody (12148-1-AP)

    IP experiment of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    IP result of anti-PRKCSH (IP:12148-1-AP, 4ug; Detection:12148-1-AP 1:1000) with HeLa cells lysate 2000ug. This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRKCSH Polyclonal antibody (12148-1-AP)

    IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRKCSH Polyclonal antibody (12148-1-AP)

    IHC staining of human kidney using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PRKCSH Polyclonal antibody (12148-1-AP)

    IHC staining of human colon using 12148-1-AP (same clone as 12148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 12148-1-AP (PRKCSH antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRKCSH Polyclonal antibody (12148-1-AP)

    IF Staining of HeLa using 12148-1-AP (same clone as 12148-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12148-1-AP (PRKCSH antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12148-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12148-1-PBS targets PRKCSH in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKCSH fusion protein Ag2796 相同性解析による交差性が予測される生物種
Full Name protein kinase C substrate 80K-H
Calculated molecular weight 80 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC013586
Gene Symbol PRKCSH
Gene ID (NCBI) 5589
RRIDAB_2253107
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP14314
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PRKCSH encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Defects in PRKCSH are a cause of an autosomal dominant polycystic liver disease (PCLD). Glucosidase II is an ER-localized enzyme that contains α and β subunits (Glucosidase IIα and Glucosidase IIβ) which form a defined heterodimeric complex.