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  • Featured Product
  • KD/KO Validated

PKC Zeta Polyclonal antibody

PKC Zeta Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10860-1-AP

Synonyms

PRKCZ, Protein kinase C zeta type, PKC2, nPKC-zeta, EC:2.7.11.13

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PKC Zeta Polyclonal antibody (10860-1-AP)

    WB analysis of HEK-293 using 10860-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PKC Zeta Polyclonal antibody (10860-1-AP)

    WB analysis of NIH/3T3 using 10860-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10860-1-AP (PKC zeta antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PKC Zeta Polyclonal antibody (10860-1-AP)

    IHC staining of human breast cancer using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PKC Zeta Polyclonal antibody (10860-1-AP)

    IHC staining of human breast cancer using 10860-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10860-1-AP (PKC zeta antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using PKC Zeta Polyclonal antibody (10860-1-AP)

    IF Staining of NIH/3T3 using 10860-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PKC Zeta antibody (10860-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10860-1-AP targets PKC Zeta in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PKC Zeta fusion protein Ag1310 相同性解析による交差性が予測される生物種
Full Name protein kinase C, zeta
Calculated molecular weight 80 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC014270
Gene symbol PKC Zeta
Gene ID (NCBI) 5590
RRIDAB_2300348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PKC Zeta antibody 10860-1-APDownload protocol
IHC protocol for PKC Zeta antibody 10860-1-APDownload protocol
IF protocol for PKC Zeta antibody 10860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Rep

aPKCζ-dependent Repression of Yap is Necessary for Functional Restoration of Irradiated Salivary Glands with IGF-1.

Authors - Alejandro M Chibly
  • KO Validated
View Article
human,mouseWB,IHC

Cancers (Basel)

Hypermethylation of PRKCZ Regulated by E6 Inhibits Invasion and EMT via Cdc42 in HPV-Related Head and Neck Squamous Cell Carcinoma

Authors - Hao-Fan Wang
  • KD Validated
View Article