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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKD3 Polyclonal antibody

PRKD3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12785-1-AP

Synonyms

EPK2, PKC NU, PKD3, PRKCN, PRKD3, Protein kinase C nu type, protein kinase D3, Protein kinase EPK2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKD3 Polyclonal antibody (12785-1-AP)

    WB analysis using 12785-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRKD3 Polyclonal antibody (12785-1-AP)

    WB analysis using 12785-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using PRKD3 Polyclonal antibody (12785-1-AP)

    WB analysis of DU 145 using 12785-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 12785-1-AP (PRKD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PRKD3 Polyclonal antibody (12785-1-AP)

    IP experiment of HEK-293 using 12785-1-AP

    IP result of anti-PRKD3 (IP:12785-1-AP, 4ug; Detection:12785-1-AP 1:300) with HEK-293 cells lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRKD3 Polyclonal antibody (12785-1-AP)

    IHC staining of human breast cancer using 12785-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRKD3 Polyclonal antibody (12785-1-AP)

    IHC staining of human breast cancer using 12785-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRKD3 Polyclonal antibody (12785-1-AP)

    IHC staining of human colon cancer using 12785-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRKD3 Polyclonal antibody (12785-1-AP)

    IHC staining of human colon cancer using 12785-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12785-1-AP (PRKD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, NIH/3T3 cells, C2C12 cells, Raji cells, Ramos cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12785-1-AP targets PRKD3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKD3 fusion protein Ag3499 相同性解析による交差性が予測される生物種
Full Name protein kinase D3
Calculated molecular weight611aa,68 kDa; 100 kDa; 890aa
Observed molecular weight 68 kDa, 100 kDa
GenBank accession numberBC030706
Gene symbol PRKD3
Gene ID (NCBI) 23683
RRIDAB_2877881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRKD3 antibody 12785-1-APDownload protocol
IHC protocol for PRKD3 antibody 12785-1-APDownload protocol
IP protocol for PRKD3 antibody 12785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,IP

Exp Mol Med

Clusterin negatively modulates mechanical stress-mediated ligamentum flavum hypertrophy through TGF-β1 signaling

Authors - Chunlei Liu
View Article
humanWB

NAR Cancer

Estrogen receptor alpha mutations regulate gene expression and cell growth in breast cancer through microRNAs

Authors - Spencer Arnesen
View Article