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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKRA Monoclonal antibody (68518-1-Ig)

    WB analysis using 68518-1-Ig (same clone as 68518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68518-1-Ig (PRKRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PRKRA Monoclonal antibody (68518-1-Ig)

    IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PRKRA Monoclonal antibody (68518-1-Ig)

    IHC staining of human ovary cancer using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRKRA Monoclonal antibody (68518-1-Ig)

    IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRKRA Monoclonal antibody (68518-1-Ig)

    IHC staining of mouse brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PRKRA Monoclonal antibody (68518-1-Ig)

    IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PRKRA Monoclonal antibody (68518-1-Ig)

    IHC staining of rat brain using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat cerebellum tissue using PRKRA Monoclonal antibody (68518-1-Ig)

    IHC staining of rat cerebellum using 68518-1-Ig (same clone as 68518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 68518-1-Ig (PRKRA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68518-1-PBS in a different storage buffer formulation.

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PRKRA Monoclonal antibody, PBS Only

PRKRA Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 68518-1-PBS
Clone No.1D4C3

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

PACT, Interferon-inducible double-stranded RNA-dependent protein kinase activator A, HSD-14, HSD14, HSD 14

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68518-1-PBS targets PRKRA in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PRKRA fusion protein Ag29818 相同性解析による交差性が予測される生物種
Full Name protein kinase, interferon-inducible double stranded RNA dependent activator
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC009470
Gene Symbol PRKRA
Gene ID (NCBI) 8575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75569
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).