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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRKX/PRKY Polyclonal antibody

PRKX/PRKY Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 17985-1-AP

Synonyms

PRKY, protein kinase, Y linked

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRKX/PRKY Polyclonal antibody (17985-1-AP)

    WB analysis using 17985-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17985-1-AP (PRKX/PRKY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inA549 cells, MCF-7 cells, NIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17985-1-AP targets PRKX/PRKY in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRKX/PRKY fusion protein Ag12404 相同性解析による交差性が予測される生物種
Full Name protein kinase, Y-linked
Calculated molecular weight 277 aa, 32 kDa
Observed molecular weight 41 kDa
GenBank accession numberBC074852
Gene symbol PRKY
Gene ID (NCBI) 5616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRKY is a member of the cAMP-dependent serine-threonine protein kinase gene family, which appears to encode a protein related to the catalytic subunit of the cAMP-dependent protein kinases, which are key players in the cellular responses to the second messenger cAMP. A chromosomal aberration involving PRKY is a cause of sex reversal disorder. As a homolog with PRKX, the antibody can recognize endogenous levels of PRKY and PRKX.

Protocols

Product Specific Protocols
WB protocol for PRKX/PRKY antibody 17985-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols