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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human pituitary tissue using Prolactin Polyclonal antibody (16525-1-AP)

    IHC staining of human pituitary using 16525-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 16525-1-AP (Prolactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Prolactin Polyclonal antibody (16525-1-AP)

    IHC staining of human pituitary using 16525-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 16525-1-AP (Prolactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using Prolactin Polyclonal antibody (16525-1-AP)

    IHC staining of rat testis using 16525-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 16525-1-AP (Prolactin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat testis tissue using Prolactin Polyclonal antibody (16525-1-AP)

    IHC staining of rat testis using 16525-1-AP

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 16525-1-AP (Prolactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using Prolactin Polyclonal antibody (16525-1-AP)

    IHC staining of mouse testis using 16525-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16525-1-AP (Prolactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pituitary tissue using Prolactin Polyclonal antibody (16525-1-AP)

    IHC staining of human pituitary using 16525-1-AP

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 16525-1-AP (Prolactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of COS-7 cells using Prolactin Polyclonal antibody (16525-1-AP)

    IF Staining of COS-7 using 16525-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed COS-7 cells using Prolactin antibody (16525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

Prolactin Polyclonal antibody

Prolactin Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 16525-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, ELISA

Synonyms

PRL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman pituitary tissue, rat testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCOS-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

16525-1-AP targets Prolactin in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Prolactin fusion protein Ag9764 相同性解析による交差性が予測される生物種
Full Name prolactin
Calculated molecular weight 227 aa, 26 kDa
GenBank accession numberBC015850
Gene Symbol Prolactin/PRL
Gene ID (NCBI) 5617
RRIDAB_2935439
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01236
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Prolactin is also named as PRL and belongs to the somatotropin/prolactin family. The proteins encoded by PRL are secreted into the cell surroundings. And they are abundantly expressed in pituitary gland, adenohypophysis, decidua and testis. Indeed, chemically, prolactin appears in a multiplicity of posttranslational forms ranging from size variants to chemical modifications such as phosphorylation or glycosylation. It is not only synthesized in the pituitary gland, as originally described, but also within the central nervous system, the immune system, the uterus and its associated tissues of conception, and even the mammary gland itself (PMID: 11015620). Prolactin acts primarily on the mammary gland by promoting lactation (PMID: 30546056). The major form of prolactin found in the pituitary gland is 23 kDa, variants of prolactin have been characterized in many mammals, including humans. Of the cleaved forms that have been characterized, 14 kDa, 16 kDa, and 22 kDa prolactin variants have been most widely studied (PMID: 7937959) (PMID: 8425495).

Protocols

Product Specific Protocols
IHC protocol for Prolactin antibody 16525-1-APDownload protocol
IF protocol for Prolactin antibody 16525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Cancer Res

Prolactin (PRL), placenta growth factor (PIGF) and nerve growth factor receptor (NGFR) as biomarkers for early diagnosis and prognosis in patients with esophageal squamous cell carcinoma (ESCC)

Authors - Xiaohong Ai
View Article