PRM1 Polyclonal antibody
PRM1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF-P, ELISA
Conjugate
Unconjugated
Cat no : 15697-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue, mouse testis tissue, rat testis tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IF | See 1 publications below |
Product Information
15697-1-AP targets PRM1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PRM1 fusion protein Ag8323 相同性解析による交差性が予測される生物種 |
| Full Name | PRM1 |
| Calculated molecular weight | 51 aa, 6 kDa |
| Observed molecular weight | 13-20 kDa |
| GenBank accession number | BC003673 |
| Gene symbol | PRM1 |
| Gene ID (NCBI) | 5619 |
| RRID | AB_2878168 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRM1 antibody 15697-1-AP | Download protocol |
| IHC protocol for PRM1 antibody 15697-1-AP | Download protocol |
| IF protocol for PRM1 antibody 15697-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Environ Pollut Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice. | ||
Theriogenology Eif2s3y alleviated LPS-induced damage to mouse testis and maintained spermatogenesis by negatively regulating Adamts5 | ||
Reprod Sci Sertoli Cell-Conditioned Medium Induces Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells to Male Germ-Like Cells in Busulfan-Induced Azoospermic Mouse Model | ||
Eur J Pharmacol 5- methylcytidine effectively improves spermatogenesis recovery in busulfan-induced oligoasthenospermia mice | ||
Cell Rep Med Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chongil (Verified Customer) (08-20-2021) | Prm1 antibody may detect a wrong protein. Prm1 is detected in elongatid spermatids and testicular sperm heads. However, this antibody detected in spermatocytes, round spermatids, and sperm tails but not in sperm heads.
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