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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRM1 Polyclonal antibody, PBS Only

PRM1 Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15697-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRM1 Polyclonal antibody (15697-1-AP)

    WB analysis using 15697-1-AP (same clone as 15697-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using PRM1 Polyclonal antibody (15697-1-AP)

    WB analysis of mouse testis using 15697-1-AP (same clone as 15697-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat testis tissue using PRM1 Polyclonal antibody (15697-1-AP)

    WB analysis of rat testis using 15697-1-AP (same clone as 15697-1-PBS)

    rat testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRM1 Polyclonal antibody (15697-1-AP)

    IHC staining of mouse testis using 15697-1-AP (same clone as 15697-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15697-1-AP (PRM1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PRM1 Polyclonal antibody (15697-1-AP)

    IF Staining of mouse testis using 15697-1-AP (same clone as 15697-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRM1 antibody (15697-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15697-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15697-1-PBS targets PRM1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRM1 fusion protein Ag8323 相同性解析による交差性が予測される生物種
Full Name PRM1
Calculated molecular weight 51 aa, 6 kDa
Observed molecular weight13-20 kDa
GenBank accession numberBC003673
Gene symbol PRM1
Gene ID (NCBI) 5619
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)